The temperature-sensitive (TS) paralyzed and blind Drosophila mutant rolling blackout (rbo) identifies a gene encoding a protein that pioneers a novel family of membrane-associated putative lipolytic enzymes highly conserved from yeast to human. RBO protein is enriched in synapse-dense neuropil and predominantly localized in presynaptic boutons at neuromuscular junction synapses, but undetectable in central neuronal cell bodies. The protein is also abundant throughout sensory neurons, including retinal photoreceptors.
RBO protein is acutely required for both phototransduction and synaptic transmission. rbo TS mutants show a reversible loss of phototransduction in an activity-dependent manner, in correlation with an activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate (PIP) and phosphatidylinositol 4,5 bisphosphate (PIP2) within minutes at non-permissive temperature. These results suggest a rapid down-regulation of phospholipase C (PLC) activity. rbo TS mutants also show reversible TS paralysis and complete block of both central and peripheral synaptic transmission within minutes at non-permissive temperature. This loss of synaptic transmission, at least partially, is due to a block of synaptic vesicle (SV) exocytosis downstream of SV docking. The conclusion is that RBO putative lipase may regulate the PLC-PIP2 signaling in both phototransduction and synaptic transmission.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03302006-055537 |
| Date | 04 April 2006 |
| Creators | Huang, Fude |
| Contributors | Douglas McMahon, Todd Graham, H. Alex Brown, Randy D. Blakely, Kendal S. Broadie |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03302006-055537/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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