Structural, biological, and phenotypic analyses of proteins that are involved in transcriptional regulation in the pathogenic bacterium Helicobacter pylori are presented. A high-resolution structure of the C-terminal domain of the รก subunit of RNA polymerase, determined using nuclear magnetic resonance methods, is presented, along with a model showing species-specific differences compared to the Escherichia coli protein. The structure of the protein encoded by the HP0564 gene is also presented, suggesting that it is a novel transcriptional regulator in H. pylori. In order to determine the specific functions of HP0564 and HP0222, another novel transcriptional regulator discovered in our laboratory, several experimental approaches were used to analyze mutant strains with disrupted HP0222 or HP0564 genes. Based on results of growth and motility assays of our mutant strains, as well as microarray data, we propose that HP0222 is a regulator of motility in H. pylori and that it may play an important role in the adhesion response.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-12102008-153314 |
| Date | 30 December 2008 |
| Creators | Borin, Brendan Nathaniel |
| Contributors | Gerald Stubbs, Charles R. Sanders, Brandt F. Eichman, Timothy L. Cover, Andrzej M. Krezel |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-12102008-153314/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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