Recently, much evidence has been brought forth into the scientific community supporting the idea that RNA Polymerase III transcribed regions of DNA may serve as chromosomal landmarks for silencing. Transfer RNA genes are known to involve themselves in several extra-transcriptional functions within the chromosome, including the pausing of replication forks, Ty element integration, tRNA position effects (repression of neighboring genes), acting as a barrier to the spread of heterochromatin, and over-riding nuclosome positioning sequences. Our results suggest that many tRNA genes may serve these functions as well as exhibiting behavior similar to metazoan insulators. Also, ETC (Extra TFIIIC) sites within Saccharomyces cerevisiae, which bind only TFIIIC may also act as barriers or insulators. Our results support the idea that extra-transcriptional functions of RNA Polymerase III factors may be widespread and important contributors to genome biology.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07102006-155549 |
| Date | 11 July 2006 |
| Creators | Simms, Tiffany Anne |
| Contributors | Patrick DiMario, David Donze, John R. Battista, Craig M. Hart |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07102006-155549/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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