Blood transfusion is a very common procedure. Before a blood transfusion it is important to find compatible blood for the patient. If the blood is incompatible with that of the patient a transfusion reaction may occur, which can be mortal. It is also important to avoid alloimmunisation. Alloimmunisation occurs when antibodies are produced against a specific antigen. These antibodies are called irregular antibodies and they can be produced after a transfusion, pregnancy, or transplantation. Alloimmunisation makes it harder to find compatible blood for patients in the future and it is a major concern for patients who require blood transfusions repeatedly. By phenotyping erythrocyte antigens, it is easier to find compatible blood for patients before a blood transfusion. Until now, a manual method has been used for phenotyping erythrocyte antigen at the Sundsvall County hospital and an automatization of this method was desired. For this study, 99 anonymised blood doner tests were used. The erythrocyte antigens M, Jka, Jkb, Fya, Fyb, S and s were phenotyped both manually and automated with ORTHO Vision Analyzer. A Clopper Pearson test was used to evaluate the accordance between the methods. A comparison was also made in regard totime and cost. The results showed a good accordance between the methods. The automated procedure using ORTHO Vision has several advantages over the manual procedure using gel cards. The risk of errors is reduced, there are fewer manual steps, it is faster and the personnel can do other tasks while the instrument is processing the tests.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-451505 |
| Date | January 2021 |
| Creators | Baranova, Valentina |
| Publisher | Uppsala universitet, Institutionen för medicinsk cellbiologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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