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Evaluation of a genomic work flow for the detection of Bacillus subtilis in animal feed and food samples

<p>Bacillus anthracis is one of the most feared agents of biological warfare and causes the</p><p>deadly disease called anthrax. SVA (statens veterinärmedicinska anstalt) is working on a</p><p>project together with SLV (statens livsmedelsverk) where the target is to find rapid and</p><p>effective detection methods for Bacillus anthracis in animal feed and food samples. Bacillus</p><p>subtilis, which is harmless, was used in this study as a model organism to Bacillus anthracis.</p><p>A known concentration of vegetative Bacillus subtilis was spiked in animal feed and food</p><p>samples. The genomic work flow was based on automated DNA isolation and real time PCR.</p><p>The aim of the study was to screen for inhibitory components in the animal feed and food</p><p>samples using two different DNA isolation robots; Magnatrix 8000 and Biorobot EZ1. The</p><p>results showed that DNA of high quality was extracted from the samples with both robots.</p><p>However, the CT-value generated by the real time PCR showed considerable variation</p><p>depending on the sample matrix. Some samples, for instance egg and liver, were problematic</p><p>and gave low concentrations and high CT-values probably due to inhibitory components in the</p><p>samples. Further studies will be needed to solve these problems and optimize the methods that</p><p>were used in this study.</p>

Identiferoai:union.ndltd.org:UPSALLA/oai:DiVA.org:uu-6345
Date January 2005
CreatorsLindberg, Stina
PublisherUppsala University, Department of Medical Biochemistry and Microbiology
Source SetsDiVA Archive at Upsalla University
LanguageEnglish
Detected LanguageEnglish
TypeStudent thesis, text

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