Neuroblastomas are the most common extra-cranial abnormal growths in immature nerve tissue within the sympathetic nervous system, in children ages 0-5. It is a heterogeneous malignancy, often emerging in and around adrenal glands, caused by rapid growth and division of nerve cells. From a clinical perspective it is difficult to treat as it has spontaneous regression, rapid progression and therapy resistance. MicroRNAs (miRNA) are small, single stranded RNA, 18-25 nucleotides long and act as human gene expression regulators which are deregulated in Neuroblastoma. The expression of miRNAs can be analysed to determine the progressive stage at which the neuroblastoma is, and the best course of treatment can be decided. The aim of this study was to analyse the expression of miRNA hsa-miR-34c-5p, in three different cell lines each having different genetic characteristics, NB69 without MYCN amplification and 11 q deletion, SN-BE with MYCN amplification and Kelly with 11q deletion. As hsa-miR-34c-5p has been found to have tumour suppressive capabilities in Neuroblastoma cell lines. The cell lines were cultured, the total RNA was isolated, Reverse Transcription was done to convert small RNA to cDNA and qPCR was run and Cq values were obtained. The miRNA expression was determined using qPCR and Livak´s method, however, no statistical difference was found in the expression of hsa-miR-34c-5p between the Neuroblastoma cell lines. These results are only a steppingstone in future research of miRNA and Neuroblastoma.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:his-22139 |
| Date | January 2022 |
| Creators | Trissl, Lorena |
| Publisher | Högskolan i Skövde, Institutionen för biovetenskap |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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