Leung Kwok-ming, Victor. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 74-78). / Chapter 1. --- SUMMARY --- p.1 / Chapter 2. --- INTRODUCTION / Chapter 2.1 --- Glycolysis : magnitude of the problem --- p.2 / Chapter 2.2 --- Glycolysis : individual variations --- p.2 / Chapter 2.2.1 --- Erythrocyte --- p.2 / Chapter 2.2.2 --- Leucocyte --- p.3 / Chapter 2.2.3 --- Age of individual --- p.3 / Chapter 2.3 --- Methods of blood glucose preservation --- p.3 / Chapter 2.3.1 --- Sodium fluoride --- p.4 / Chapter 2.3.1.1 --- Delayed effectiveness of NaF ; clinical implications --- p.6 / Chapter 2.3.2 --- D-mannose --- p.7 / Chapter 2.3.3 --- Citric acid --- p.9 / Chapter 2.3.4 --- Cooling --- p.10 / Chapter 2.3.5 --- Haemolysis --- p.11 / Chapter 2.4 --- Aims of the project --- p.12 / Chapter 3. --- MATERIALS & METHODS / Chapter 3.1 --- Evaluation of the YSI 23 AM glucose analyser for the measurement of glucose in haemolysate --- p.15 / Chapter 3.1.1 --- Principle of measurement --- p.15 / Chapter 3.1.2 --- Preparation of haemolysate --- p.16 / Chapter 3.1.3 --- Procedure --- p.16 / Chapter 3.2 --- Development of a glucose assay in haemolysate in Cobas Bio and Cobas Mira --- p.17 / Chapter 3.2.1 --- Glucose measurement --- p.17 / Chapter 3.2.2 --- Preparation of haemolysate --- p.17 / Chapter 3.2.3 --- Cobas Bio --- p.17 / Chapter 3.2.3.1 --- Optimisation of sample size --- p.17 / Chapter 3.2.3.2 --- Discussion --- p.21 / Chapter 3.2.4 --- Cobas Mira --- p.22 / Chapter 3.2.4.1 --- Optimisation of sample/reagent volume ratio --- p.22 / Chapter 3.2.4.2 --- Discussion --- p.22 / Chapter 3.2.4.3 --- Optimisation of the assay protocol --- p.26 / Chapter 3.2.4.4 --- Evaluation of the RS-SR1 protocol --- p.34 / Chapter 3.2.4.5 --- Discussion --- p.36 / Chapter 3.2.5 --- To study the effect of variations in haematological parameters on the re- covery of glucose inhaemolysate --- p.36 / Chapter 3.2.5.1 --- Procedure / :Experiment (i) --- p.36 / :Experiment (ii) --- p.43 / Chapter 3.2.5.2 --- Discussion --- p.48 / Chapter 3.2.6 --- Interference study of saponin --- p.50 / Chapter 3.2.6.1 --- Procedure --- p.50 / Chapter 3.2.6.2 --- Discussion --- p.50 / Chapter 3.2.7 --- Interference study of SDS --- p.53 / Chapter 3.2.7.1 --- Procedure --- p.53 / Chapter 3.2.7.2 --- Discussion --- p.53 / Chapter 3.3 --- Stability of glucose in haemolysate --- p.55 / Chapter 3.3.1 --- Preparation of haemolysate --- p.55 / Chapter 3.3.2 --- Glucose measurement --- p.55 / Chapter 3.3.3 --- Procedure --- p.55 / Chapter 3.3.3.1 --- Experiment (i) --- p.55 / Chapter 3.3.3.2 --- Experiment (ii) --- p.55 / Chapter 3.3.4 --- Statistical analysis --- p.56 / Chapter 4. --- RESULTS / Chapter 4.1 --- Evaluation of the YSI 23 AM glucose analyser for the measurement of glucose in haemolysate --- p.57 / Chapter 4.2 --- Stability of glucose in haemolysate --- p.60 / Chapter 5. --- DISCUSSION --- p.66 / Chapter 6. --- ACKNOWLEDGMENT --- p.73 / Chapter 7. --- REFERENCES --- p.74
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318913 |
| Date | January 1992 |
| Contributors | Leung, Kwok-ming., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 78 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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