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Isolation and Partial Characterization of Lecithin Cholesterol Acyltransferase and High Density Lipoprotein from Hog Plasma

Lecithin:cholesterol acyltransferase (LCAT) was purified 30,000-fold from hog plasma in a homogeneous state as indicated by polyacrylamide gel electrophoresis. The purified enzyme had an apparent molecular weight of 66,000 and was found to contain about 21.4 percent (w/w) carbohydrate. The properties of hog LCAT including amino acid composition were compared with human LCAT. High density lipoprotein (HDL) was isolated from the hog plasma by an immunoaffinity column chromatography. The isolated HDL showed nearly identical lipid-protein composition although it contained additional protein components when it was compared to HDL isolated by a traditional method involving ultracentrifugation.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc504140
Date05 1900
CreatorsPark, Yong Bok
ContributorsLacko, Andras G., Gracy, Robert W., Harris, Ben G., Jacobson, Myron
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvi, 78 leaves: ill., Text
RightsPublic, Park, Yong Bok, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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