C3d is a sub-fragment of the C3 component of the complement system. Covalent binding of multiple C3ds to antigen reduces the activation threshold of cognate B lymphocytes by one thousand fold through co-ligation of the BCR and complement receptor 2 (CR2/CD21). Reverse transcriptase polymerase chain reaction (RT-PCR), revealed that, in cattle, four distinct complement receptors are produced from the Cr2 gene by alternative splicing. Cattle express two major variants of the Cr2 gene representing homologues of murine CR1 and CR2, each of which are expressed in both a long and a short form. Expression of CR1 was detected in splenocytes but not in splenic mononuclear cells or monocyte derived macrophages. CR2 was detected only on IgM+ blood cells and unsorted splenocytes but not in CD3+ cells, CD14+ cells or neutrophils. Additionally, the coding sequence of CD19, the CR2 co-signaling molecule was found.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OGU.10214/2794 |
| Date | 20 June 2011 |
| Creators | Pringle, Eric S. |
| Contributors | Shewen, Patricia E. |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | http://creativecommons.org/licenses/by-nc-nd/3.0/ |
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