Olfactory Granule cells (GCs) are a population of inhibitory interneurons
responsible for maintaining normal olfactory bulb (OB) function and circuitry. Through
dendrodendritic synapses with the OBs projection neurons, the GCs regulate information
sent to the olfactory cortices. Throughout adulthood, GCs continue to integrate into the OB
and contribute to olfactory circuitry. However, only ~50% will integrate and survive longterm.
Factors aiding in the survival and morphological development of these neurons are
still being explored. The neurotrophin brain-derived neurotrophic factor (BDNF) aids in
the survival and dendritic spine maturation/maintenance in several populations of CNS
neurons. Investigators show that increasing BDNF in the adult-rodent SVZ stimulates
proliferation and increases numbers of new OB GCs. However, attempts to replicate these
experiments failed to find that BDNF affects proliferation or survival of adult-born granule
cells (abGCs). BDNFs regulation of dendritic spines in the CNS is well characterized. In
the OB, absence of BDNF’s receptor on abGCs hinders normal spine development and demonstrates a role for BDNF /TrkB signaling in abGCs development. In this study, we
use transgenic mice over-expressing endogenous BDNF in the OB (TgBDNF) to determine
how sustained increased in BDNF affect the morphology of olfactory GCs and the survival
and development of abGCs. Using protein assays, we discovered that TgBDNF mice have
higher BDNF protein levels in their OB. We employed a Golgi-cox staining technique to
show that increased BDNF expression leads to an increase in dendritic spines, mainly the
mature, headed-type spine on OB GCs. With cell birth-dating using 5-bromo-2’-
deoxyuridine (BrdU), immunofluorescent cell markers, TUNEL staining and confocal
microscopy, we demonstrate that over-expression of BDNF in the OB does not increase
survival of abGCs or reduce cell death in the GC population. Using virally labeled abGCs,
we concluded that abGCs in TgBDNF mice had similar integration patterns compared to
wild-type (WT) mice, but maintained increases in apical headed-type spine density from
12 to 60 days PI. The evidence combined demonstrates that although increased BDNF does
not promote cell survival, BDNF modifies GC morphology and abGC development
through its regulation of dendritic spine development, maturation and maintenance in vivo. / Includes bibliography. / Dissertation (Ph.D.)--Florida Atlantic University, 2018. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_40939 |
| Contributors | McDole, Brittnee (author), Guthrie, Kathleen (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Science, Department of Biological Sciences |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 163 p., application/pdf |
| Rights | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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