Liang, Fengting. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 87-101). / Abstracts in English and Chinese. / DECLARATION --- p.I / ACKNOWLEDGEMENT --- p.II / ABBREVIATIONS --- p.III / ABSTRACT IN ENGLISH --- p.IV / ABSTRACT IN CHINESE --- p.VI / TABLE OF CONTENTS --- p.VIII / LIST OF FIGURES --- p.XI / LIST OF TABLES --- p.XIII / Chapter CHAPTER I- --- INTRODUCTION / Chapter 1.1 --- Roles of human bronchial epithelial cells --- p.1 / Chapter 1.2 --- Role of epithelium in airway inflammation --- p.3 / Chapter 1.3 --- Pathology of asthma --- p.5 / Chapter 1.4 --- The role of eosinophils in asthma --- p.7 / Chapter 1.5 --- "Effects of poly-L-arginine, a MBP analogue, on airway epithelium" --- p.10 / Chapter 1.6 --- Inflammatory pathways involved in epithelial cytokine production --- p.12 / Chapter 1.7 --- Roles and function of IL-6 and IL-8 in epithelial cells --- p.16 / Chapter 1.8 --- P2 receptors and inflammation --- p.18 / Chapter 1.9 --- Objectives --- p.20 / Chapter CHAPTER II- --- MATERIALS AND METHODS / Chapter 2.1 --- Materials and regents --- p.21 / Chapter 2.2 --- Cell culture --- p.22 / Chapter 2.3 --- RNA extraction and Real-time PCR --- p.23 / Chapter 2.4 --- Measurement of cytokine secretion by antibody array --- p.24 / Chapter 2.5 --- Quantification of IL-6 and IL-8 secretion --- p.27 / Chapter 2.6 --- Western Blotting --- p.28 / Chapter 2.7 --- NF-kB translocation assay --- p.29 / Chapter 2.8 --- Data analysis --- p.30 / Chapter CHAPTER III- --- RESULTS / Chapter 3.1 --- Poly-L-arginine-induced IL-6 and IL-8 release from 16HBE 14o- --- p.31 / Chapter 3.2 --- Signaling pathways involved in poly-L-arginine-induced IL-6 and IL-8 release --- p.34 / Chapter 3.2.1 --- "Effects of p38 MAPK, ERK1/2 and NF-kB inhibitors on poly-L-arginine-induced IL-6 and IL-8 release" --- p.35 / Chapter 3.2.2 --- Poly-L-arginine induces p38 MAPK and ERK1/2 phosphorylation --- p.43 / Chapter 3.2.3 --- Poly-L-arginine activates NF-kB translocation from cytoplasm to nucleus --- p.49 / Chapter 3.3 --- Effects of MAPK and NF-kB inhibitors on IL-6 and IL-8 mRNA expression on poly-L-arginine-challenged 16HBE14o- cells --- p.52 / Chapter 3.4 --- P2 receptors modulate poly-L-arginine-induced IL-6 and IL-8 ^ production --- p.55 / Chapter 3.4.1 --- Extracellular nucleotides modulate IL-6 and IL-8 production --- p.56 / Chapter 3.4.2 --- Effects of P2Y6 antagonist on poly-L-arginine-induced IL-6 and IL-8 production --- p.61 / Chapter 3.4.3 --- Effects of MAPKs inhibitors on UDP-induced IL-6 and IL-8 secretion --- p.64 / Chapter 3.4.4 --- UDP induces NF-kB translocation in 16HBE14o- cells --- p.67 / Chapter CHAPTER IV- --- DISCUSSION / Chapter 4.1 --- Involvement of p3 8 MAPK and NF-kB in poly-L-arginine-induced IL-6 and IL-8 secretion --- p.70 / Chapter 4.2 --- Involvement of p38 MAPK and NF-kB in poly-L-arginine-induced IL-6 and IL-8 mRNA elevation --- p.72 / Chapter 4.2.1 --- Regulation of NF-kB on IL-6 and IL-8 mRNA --- p.73 / Chapter 4.2.2 --- Regulation of p38 MAPK on IL-6 and IL-8 mRNA --- p.75 / Chapter 4.2.3 --- Crosstalk between NF-kB and p38 MAPK --- p.77 / Chapter 4.3 --- Extracellular nucleotides mediate IL-6 and IL-8 production in 16HBE14o- --- p.79 / Chapter 4.3.1 --- P2Y6 receptor is linked to poly-L-arginine-induced IL-6 and IL-8 release --- p.80 / Chapter 4.3.2 --- P2Y6 receptor regulates IL-6 and IL-8 secretion via p38 MAPK and NF-kB --- p.83 / Chapter 4.4 --- Summary --- p.86 / Chapter CHAPTER V- --- References --- p.87 / Publications --- p.102
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327132 |
| Date | January 2010 |
| Contributors | Liang, Fengting., Chinese University of Hong Kong Graduate School. Division of Biomedical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 102 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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