The Feldmannia sp. virus is a large icosahedral virus that persistently infects
marine brown alga Feldmannia sp.. So far, there is no information available about
viral genome replication, gene structure and gene expression in this unique viral-host
system. The purpose of this study was to characterize the general features of viral
transcripts in the virus producing sporophyte plants. Northern analysis, using four
cosmid clones that cover the entire viral genome, showed that there were six major
transcripts and at least eighteen minor transcripts in the virus producing sporophyte
plants. These transcripts are not evenly distributed in the viral genome. A 5.7 kb
BamHI fragment-R was found to encode a 1.5 kb and a 0.9 kb major transcript, and
those two major transcripts were chosen for detailed sequence analysis. The 1.5 kb
transcript was identified as the putative major capsid protein (MCP) gene. The FsV
MCP has significant similarity with the major capsid protein of Chlorella virus-PBCV-1 and with iridoviruses, fish lymphocystis disease virus, frog virus 3, and with African
swine fever virus. / Graduation date: 1996
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/34633 |
| Date | 26 April 1996 |
| Creators | Jia, Yibing |
| Contributors | Meints, Russel H. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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