by Bi Yu Rong. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 188-220). / Acknowledgment --- p.i / Abstract --- p.ii / Table and contents --- p.v / List of abbreviation --- p.x / List of Tables --- p.xi / List of Figures --- p.xiv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.5 / Chapter 2.1 --- General information of plant growth regulators --- p.5 / Chapter 2.2 --- Natural plant growth inhibitors --- p.14 / Chapter 2.3 --- Alkaloids and narciclasine --- p.15 / Chapter 2.4 --- Studies on expansion and greening of cotyledons --- p.22 / Chapter 2.5 --- Investigation on chlorophyll synthesis --- p.28 / Chapter Chapter 3 --- Materials and methods --- p.31 / Chapter 3.1 --- Plant materials --- p.31 / Chapter 3.2 --- Isolation and purification of inhibitory substance from Narcissus bulbs --- p.31 / Chapter I. --- Isolation of inhibitory substance from fresh Narcissus bulbs --- p.31 / Chapter II. --- Partial purification of the inhibitory substance with different organic solvents --- p.32 / Chapter III. --- Purification and identification --- p.32 / Chapter A. --- Thin layer chromatography (TLC) --- p.32 / Chapter B. --- Column chromatography --- p.33 / Chapter C. --- Spectrometric analyses --- p.33 / Chapter IV. --- Bioassays --- p.34 / Chapter 3.3 --- Effect of narciclasine (NCS) on the seeds germination and seedling growth --- p.34 / Chapter I. --- Germination experiments --- p.35 / Chapter II. --- Seedlings growth --- p.35 / Chapter 3.4 --- Interaction of NCS and phytohormones --- p.35 / Chapter I. --- Interaction with abscisic acid (ABA) --- p.36 / Chapter A. --- Seed germination --- p.36 / Chapter B. --- Seedling growth --- p.36 / Chapter II. --- Interaction with auxin --- p.36 / Chapter III. --- Interaction with gibberellin --- p.37 / Chapter VI. --- Interaction with cytokinin --- p.38 / Chapter 3.5 --- Interaction of NCS and phytohormones to growth and greening of excised radish cotyledons exposing to light --- p.39 / Chapter I. --- Growth of excised radish cotyledons exposing to light --- p.39 / Chapter II. --- Chlorophyll content determination --- p.40 / Chapter III. --- Effects of a pretreatment with BA or NCS on the growth and greening of excised radish cotyledons --- p.40 / Chapter 3.6 --- Effect of NCS on the growth and greening of excised radish cotyledons and etiolated wheat leaves --- p.41 / Chapter I. --- Effect of NCS on the growth and greening of excised radish cotyledons --- p.41 / Chapter II. --- Effect of NCS on the greening of etiolated wheat leaves --- p.41 / Chapter 3.7 --- Effect of NCS on chlorophyll synthesis and δ-aminolevulinic acid (ALA) accumulation of etiolated wheat leaves in presence of levulinic acid (LA) --- p.42 / Chapter 3.8 --- Enzymes studies in the excised radish cotyledons --- p.43 / Chapter I. --- Assay of isocitrate lyase activity --- p.44 / Chapter II. --- Assay of hydroxypyruvate reductase activity --- p.44 / Chapter 3.9 --- Ultrastructural studies --- p.45 / Chapter Chapter 4. --- Results --- p.47 / Chapter 4.1 --- Chemical studies of NCS --- p.47 / Chapter I. --- Isolation and partial purification of inhibitory substance from Narcissus bulbs --- p.47 / Chapter A. --- "Effect of lyophilized slimy secretion (LSS) on the germination seeds, the growth of radicle and hypocotyl of seedlings of Brassica" --- p.47 / Chapter B. --- Effect of different solvent extracts on the germination and the elongation of radicle and hypocotyl of Brassica seedlings --- p.47 / Chapter C. --- Effect of fraction isolated with n-butanol from dried bulbs or LSS on the germination of Brassica seeds and radicle growth --- p.49 / Chapter D. --- Purification of inhibitory substance from Narcissus bulbs by chromatography --- p.54 / Chapter II. --- Identification of the inhibitory substance from Narcissus bulbs .… --- p.62 / Chapter 4.2 --- Physiological and biochemical studies ofNCS --- p.70 / Chapter I. --- Effects ofNCS on seed germination and seedlings growth of Brassica --- p.70 / Chapter II. --- Time course studies ofNCS on germination and growth of radish seeds --- p.70 / Chapter III. --- Comparative studies ofNCS and ABA on seeds germination and seedlings growth --- p.73 / Chapter IV. --- Interaction between NCS and phytohormones --- p.79 / Chapter A. --- Interaction of NCS with ABA --- p.79 / Chapter B. --- Interaction of NCS with IAA --- p.84 / Chapter C. --- Interaction of NCS with gibberellin --- p.84 / Chapter D. --- Interaction of NCS with cytokinin --- p.89 / Chapter V. --- Effects ofNCS and BA on chlorophyll and carotenoid content of excised cotyledons --- p.89 / Chapter A. --- "Effects ofNCS and BA on expansion, chlorophyll content and carotenoid content of excised radish cotyledons" --- p.89 / Chapter B. --- Effects of a pretreatment with BA or NCS on the growth and greening of excised radish cotyledons --- p.97 / Chapter VI. --- Interaction between NCS and phytohormones in growth and greening of excised radish cotyledons --- p.105 / Chapter A. --- "Effects of BA,GA3 and ABA on the growth and greening of excised radish cotyledons" --- p.105 / Chapter B. --- Interaction of NCS with phytohormones on growth and greening of excised radish cotyledons --- p.108 / Chapter 4.3 --- Investigation of effects of NCS on chlorophyll synthesis --- p.113 / Chapter I. --- Effect of preincubation in water on growth and greening of excised cotyledons under light --- p.113 / Chapter II. --- Effect of NCS on the growth and greening of etiolated radish excised cotyledons --- p.116 / Chapter III. --- Effect of NCS on the greening of etiolated leaves of 7-day-old wheat seedlings under light --- p.116 / Chapter IV. --- Effect of LA on ALA accumulation in the light --- p.120 / Chapter V. --- Time course study of NCS on ALA accumulation in the presence of LA --- p.122 / Chapter 4.4 --- Effect of NCS on the development of enzymes activities in the excised radish cotyledons --- p.122 / Chapter I. --- Effect of NCS on isocitrate lyase activity of excised radish cotyledons --- p.122 / Chapter II --- Effect of NCS on hydroxypyruvate reductase activity of excised radish cotyledons --- p.125 / Chapter 4.5 --- Effect of NCS on ultrastructural changes of excised radish cotyledons --- p.128 / Chapter I. --- Time course studies --- p.128 / Chapter II. --- "Effect ofNCS, BA and ABA on the ultrastructural change of excised radish cotyledons in the light" --- p.142 / Chapter III. --- Effect of a pretreatment with dark on the inhibition ofNCS on ultrastructural change of excised radish cotyledons in light --- p.150 / Chapter Chapter 5. --- Discussion --- p.160 / Chapter Chapter 6. --- Conclusions --- p.180 / References --- p.188
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321649 |
| Date | January 1996 |
| Contributors | Bi, Yu Rong., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvii, 220 leaves : ill. (some col., some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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