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Previous issue date: 2007-09-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The adhesion molecules E-cadherin and β-catenin have been studied as possible markers to distinguish carcinomas with and without metastatic potential. The objective of this research was to study the imunohistochemistry expression of the E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC), aiming to contribute for the better understanding of the biological behavior of this lesion. The sample consisted of 30 cases of OSCC, being 15 of tongue and 15 of lower lip. The profile and intensity of labeling and semi quantitative analysis of the percentage of immunopositive tumoral cells in membrane for E-cadherin and β-catenin had been related with the anatomical localization of the lesion, the presence or not of nodal metastasis and the histological grade of malignancy in the invasive front area of the tumor. It was registered the presence or not of cytoplasmic and nuclear labeling of the β-catenin. The results had been submitted to the statistical analysis, being used the Mann-Whitney Test, the Fisher Test and the Spearman Correlation Coefficient (α=0, 05). The results showed that the expression in membrane for E-cadherin and β-catenin was, predominantly, the heterogeneous profile in the lower lip and tongue carcinomas, as well as in the cases with and without nodal metastasis. It was not observed significant statistical difference between expression profile and amount of immunopositive cells for E-cadherin, β-catenin and the anatomical localization of the lesion and for the presence or not of nodal metastasis. However, there was significant difference of the reduced expression of these proteins with the high score of malignancy. It was verified that the expression of the β-catenin in cytoplasm was present in 22 (73.33%) of the 30 analyzed cases, and 6 cases (20%) showed nuclear expression. The statistical analysis detected significant association between the expression of the β-catenin in the cytoplasm with the histological grade of malignancy, being this molecule more frequently present in the cytoplasm in the cases of high score of malignancy. It was concluded that the reduced immunoexpression of these proteins in membrane can be related with the lowest cellular differentiation, as well as with the pattern of invasion in nests and isolated cells, demonstrated in the cases of OSCC with high histological grade of malignancy / As mol?culas de ades?o E-caderina e β-catenina t?m sido estudadas como poss?veis marcadores para distinguir carcinomas com e sem potencial metast?tico. O objetivo desta pesquisa foi estudar a express?o imuno-histoqu?mica da E-caderina e β-catenina em carcinoma epiderm?ide oral (CEO), visando contribuir para uma melhor compreens?o do comportamento biol?gico desta les?o. A amostra constou de 30 casos de CEO, sendo 15 de l?ngua e 15 de l?bio inferior. O padr?o e intensidade de marca??o e a an?lise semiquantitativa do percentual de c?lulas tumorais imunopositivas em membrana para E-caderina e β-catenina foram relacionados com a localiza??o anat?mica da les?o, a presen?a ou n?o de met?stase nodal e a grada??o histol?gica de malignidade no front de invas?o tumoral. Tamb?m foi registrada a presen?a ou n?o de imunomarca??o citoplasm?tica e nuclear da β-catenina. Os resultados foram submetidos ? an?lise estat?stica, sendo utilizados o Teste de Mann-Whitney, o Teste Exato de Fisher e o Coeficiente de correla??o de Spearman (α=0,05). Os resultados mostraram que a express?o em membrana para E-caderina e β-catenina exibiram, predominantemente, o padr?o heterog?neo nos carcinomas de l?bio inferior e nos de l?ngua, assim como nos casos com e sem met?stase nodal. Aplicados os testes estat?sticos, observou-se que n?o houve diferen?a significativa entre o padr?o de express?o e a quantidade de c?lulas imunopositivas para E-caderina e β-catenina e a localiza??o anat?mica da les?o e para a presen?a ou n?o de met?stase nodal. Por?m, verificou-se diferen?a estatisticamente significativa da express?o reduzida destas prote?nas com o alto escore de malignidade. Observou-se que a express?o da β-catenina em citoplasma estava presente em 22 (73,33%) dos 30 casos analisados, sendo que em 6 casos (20%) houve a express?o em n?cleo. Ap?s a an?lise estat?stica, foi detectada uma associa??o significativa entre a express?o da β-catenina no citoplasma com a grada??o histol?gica de malignidade, estando esta mol?cula mais freq?entemente presente no citoplasma nos casos de alto escore de malignidade. Conclui-se que a imunoexpress?o reduzida destas prote?nas em membrana pode estar relacionada com o menor grau de diferencia??o celular, bem como com o padr?o de invas?o em ninhos e em c?lulas isoladas, demonstrados nos casos de CEO de alto escore
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17163 |
| Date | 28 September 2007 |
| Creators | Lopes, Fernanda Ferreira |
| Contributors | http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787927Z7&dataRevisao=null, Oliveira, M?rcio Campos, CPF:67676146534, http://lattes.cnpq.br/5703051980918880, Bastos, Eider Guimar?es, CPF:33093156300, http://lattes.cnpq.br/3285430538082061, Freitas, Roseana de Almeida, CPF:28444361453, http://lattes.cnpq.br/9512014003639405, Souza, L?lia Batista de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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