The Caliciviridae contains many viruses which are pathogenic to humans, marine
mammals, domestic animals, and numerous species of wildlife. Currently there is no single assay
to detect antigenic response to the multiple serotypes of calicivirus. The development of
calicivirus specific synthetic peptides having highly conserved epitopes common to many
serotypes would facilitate the development of a simple and rapid serologic assay for calicivirus
antibodies irrespective of the serotype. Calicivirus cDNA recombinants which express fusion
proteins that react with multiple calicivirus typing sera may be useful in the development of a
serologic assay for antibodies to caliciviruses. For this purpose RNA was isolated from cell culture
infected with San Miguel sea lion virus type 5 (SMSV-5) and used to construct a cDNA library,
named SMSV-5 lambda. Immunoassay techniques were used to screen the SMSV-5 lambda
library and a second cDNA library, named SMSV-5RT, also constructed from SMSV-5. One
recombinant named 8-SN was identified which produced a fusion protein that reacted positively
with a pool of four polyclonal calicivirus typing sera (SMSV-5, SMSV-13, SMSV-15, and SMSV-17). This construct was amplified, induced, and a fusion protein identified which reacted positively
in four western blot assays using individual polyclonal typing sera to the caliciviruses SMSV-13,
SMSV-15, SMSV-16, and SMSV-17. / Graduation date: 1997
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/34288 |
| Date | 31 July 1996 |
| Creators | Stone, Michael A. |
| Contributors | Smith, Alvin W. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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