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Effect of the calpain inhibitor E-64-d on the degradation of α-fodrin in damaged muscle

Graduation date: 2006 / We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain inhibitor. Both EDL and SOL muscles displayed significant differences (p<0.003 and p<0.002 respectively) between the raw and normalized 150 and 145 kDa α-fodrin fragments of the DMSO + E-64-d compared to the other bath treatments. Based on our model of exercise-induced muscle damage, we expected to see greater levels of 150 and 145 kDa α-fodrin fragments in those muscles that performed the eccentric protocol. However, there was no evidence that eccentric muscle damage increased the levels of 150 and 145 kDa α-fodrin fragments over the levels observed in the isometric trials. These findings suggest that the magnitude of damage was insufficient to activate calpains.

Identiferoai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/1940
Date23 May 2006
CreatorsBoyd, Jeffrey
ContributorsWidrick, Jeffrey J., Wilcox, Anthony, Bella, Deborah, Levenson, Rick
Source SetsOregon State University
Languageen_US
Detected LanguageEnglish
TypeThesis
Format464645 bytes, application/pdf

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