To better understand the nature and function of N-acylethanolamines (NAEs) in Camelina sativa, we used mass spectrometry analysis to identify and quantify NAE types in developing seeds, desiccated seeds and seedlings. Developing seeds showed a differential increase in individual NAE species and an overall increase in NAE content with seed development and maturation. The NAE composition in mature, desiccated seeds mostly reflected the total fatty acid composition in the seed tissues, except for a noted absence of 11-eicosenoic (20C monounsaturated) fatty acid in the NAE pool. During seed stratification and seedling growth, individual NAE species were depleted at similar rates. Simulated drought treatments during seedling development resulted in a significant rise in NAE levels for the major 18C NAE types compared with untreated seedlings. Arabidopsis and Camelina mutants with reported altered fatty acid profiles were analyzed for their NAE compositions; both Arabidopsis and Camelina had relatively similar changes between compositions of total seed fatty acids and NAEs. Furthermore, seeds were analyzed from transgenic Arabidopsis and Camelina with engineered, non-native, long-chain polyunsaturated fatty acids (18C, 20C and 22C), and the results showed the production of novel N-acylphosphatidylethanolamines (presumed precursors of NAEs) and NAEs with the same long acyl chains. These results demonstrate i) that NAE levels change dramatically with seed and seedling development in Camelina, ii) that NAE levels in Camelina seedlings can be elevated in response to environmental stress, and iii) that the pool of NAEs can be manipulated by making alterations to seed fatty acid compositions, some of which may have promise for the production of novel, bioactive NAEs.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc1707362 |
| Date | 08 1900 |
| Creators | Corley, Chase D |
| Contributors | Chapman, Kent D, Shah, Jyoti, Verbeck, Guido F |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 64 pages, Text |
| Rights | Public, Corley, Chase D, Copyright, Copyright is held by the author, unless otherwise noted. All rights Reserved. |
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