A phytochemical investigation on methanolic extract of roots of yellow rose led to
the isolation of a catechin gallate. The crude extract first underwent fractionation
using petroleum ether, chloroform, ethylacetate, butanol with water as solvents in
their respective order. The emerging solvent fractions were subjected to further
separation using lipophilic sephadex (LH-20), and silica gel column chromatography
to isolate pure compounds. Analytical thin layer chromatography (TLC) was used to
confirm the presence of a catechin in butanol fraction. Purified catechin compound
was subjected to 2,2-diphenyl-1-picrylhydrazyl (DPPH) experiment to determine its
Radical Scavenging Capacity, which was found quite promising. Chemical structure
of purified compound was established by using Nuclear Magnetic Resonance (NMR)
and Mass Spectrometry (MS) experiments.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/3/12610686/index.pdf |
| Date | 01 June 2009 |
| Creators | Kyejjusa, Yusuf |
| Contributors | Coruh, Nursen |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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