by Fung Loi Mo. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 78-80). / Acknowledgment --- p.1 / Summary --- p.2 / List of abbreviation --- p.4 / Index of tables --- p.6 / Index of figures --- p.7 / Chapter 1. --- Introduction --- p.9 / Chapter 2. --- Literature review / Chapter 2.1 --- Catecholamines (CATS) / Chapter 2.1.1 --- Structures and properties --- p.11 / Chapter 2.1.2 --- "Origin, biosynthesis and release" --- p.14 / Chapter 2.1.3 --- Metabolism --- p.16 / Chapter 2.1.4 --- Biological functions --- p.23 / Chapter 2.1.5 --- Significance of urinary free CATS measurement --- p.23 / Chapter 2.2 --- Methods of CATS measurement / Chapter 2.2.1 --- Colorimetric method --- p.26 / Chapter 2.2.2 --- Fluorometric method --- p.26 / Chapter 2.2.3 --- Radioenzymatic method --- p.27 / Chapter 2.2.4 --- Chromatographic method --- p.27 / Chapter 2.3 --- Method of preservation of urinary free CATS / Chapter 2.3.1 --- Reasons for preservation --- p.30 / Chapter 2.3.2 --- Common preservatives --- p.31 / Chapter 2.3.3 --- Systematic CATS preservation studies --- p.33 / Chapter 2.3.4 --- Conclusions --- p.35 / Chapter 3. --- Aim of study --- p.37 / Chapter 4. --- Materials and Methods / Chapter 4.1 --- Principle of method --- p.38 / Chapter 4.2 --- Materials --- p.39 / Chapter 4.2.1 --- Chemicals --- p.39 / Chapter 4.2.2 --- Equipment --- p.39 / Chapter 4.2.3 --- Reagent preparation for CATS analysis --- p.39 / Chapter 4.2.4 --- Reagent preparation for the different studies --- p.42 / Chapter 4.3 --- Methods / Chapter 4.3.1 --- Alumina extraction of urinary free CATS --- p.44 / Chapter 4.3.2 --- Separation and quantitation of free CATS by HPLC --- p.45 / Chapter 4.3.3 --- Statistical analysis --- p.46 / Chapter 4.4 --- Precision performance and recovery study --- p.47 / Chapter 4.5 --- Choice of buffer for preserving urinary free CATS --- p.47 / Chapter 4.6 --- Optimal concentration of citrate and formate buffer --- p.48 / Chapter 4.7 --- Effects of citrate and formate buffer on measurement of urinary free CATS --- p.50 / Chapter 4.8 --- Effects of different preservatives on urinary free CATS at 37°C --- p.52 / Chapter 4.9 --- Effects of different storage temperatures on the stability of buffered urinary free CATS --- p.52 / Chapter 5. --- Results and discussions / Chapter 5.1 --- Precision performance and recovery study --- p.56 / Chapter 5.2 --- Choice of buffer for preserving urinary free CATS --- p.58 / Chapter 5.3 --- Optimal concentration of citrate and formate buffer --- p.61 / Chapter 5.4 --- Effects of citrate and formate buffer on measurement of urinary free CATS --- p.63 / Chapter 5.5 --- Effects of different preservatives on urinary free CATS at 37°C --- p.65 / Chapter 5.6 --- Effects of different storage temperatures on the stability of buffered urinary free CATS --- p.68 / Chapter 6. --- Conclusions --- p.76 / Chapter 7. --- Limitations and future prospects --- p.77 / Chapter 8. --- References --- p.78
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321505 |
| Date | January 1996 |
| Contributors | Fung, Loi Mo., Chinese University of Hong Kong Graduate School. Division of Clinical Biochemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 80 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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