Promoter elements prediction with bioinformatics means is very promising direction in molecular biology. Such predictions allow to clarify the mechanisms of gene regulation and to increase the success of the “wet” experiments. The study was aimed to predict motifs for the 20 genes expressed in the thermosensory AFD neurons of the C.elegans and ortholog species. As theoretical basis of investigation was accepted theory about conservation of functionally important non-coding regions between ortholog genes. Some of such conserved regions can serve as the putative promoter elements. For the investigation the set of ortholog genes of the C.elegans, C.briggsae and C.remani was used. During the work suitable bioinformatics programs and the set of promoter criteria were worked out. Predicted promoters with the different level of conservation were compared to the each other and with the known regulatory elements. Some of the promoter elements (CAGCTG, CAGGTG) represent sequences are highly conserved from worms to the human others are similar to the known worm motifs (skn-1). Genes with the similar putative elements were combined in the groups with the possible common mechanisms of the gene regulation. The investigation of the putative promoter elements requires the development of new programs,methods and criteria of the evaluation. This would be appropriate strategy for the further motifs and non-coding DNA studying.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:sh-621 |
| Date | January 2005 |
| Creators | Pyshchyta, Ganna |
| Publisher | Södertörns högskola, Huddinge : Institutionen för livsvetenskaper |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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