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Early migration of cardiac neural crest cells in normal and splotch mouse embryos.

by Cheung Chui Shan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 96-107 (2nd gp.)). / Abstracts in English and Chinese. / ABSTRACT (ENGLISH) --- p.i / ABSTRACT (CHINESE) --- p.iii / ACKNOWLEDGEMENTS --- p.v / TABLE OF CONTENT --- p.vii / Chapter CHAPTER ONE --- GENERAL INTRODUCTION / Chapter 1.1 --- Early development of the central nervous system --- p.1 / Chapter 1.2 --- Neural crest cells and Cardiac neural crest cells --- p.1 / Chapter 1.3 --- Role of neural crest cells in cardiovascular development --- p.4 / Chapter 1.4 --- Methods in tracing neural crest cells --- p.7 / Chapter 1.5 --- Neural crest-related defects --- p.14 / Chapter 1.6 --- Animal models for studying neural crest defects --- p.16 / Chapter 1.7 --- Recent studies on the migration of cardiac neural crest cells in mammals --- p.19 / Chapter 1.8 --- Objectives of the present study --- p.22 / Chapter CHAPTER TWO --- MATERIALS AND METHODS / Chapter 2.1 --- Materials --- p.26 / Pregnant mice --- p.26 / Pregnant Splotch mice (Sp2H) --- p.26 / Preparation of the handling medium --- p.27 / Preparation of the culture medium --- p.27 / Gas mixtures for embryo culture --- p.30 / Preparation of wheat germ agglutinin-gold conjugates (WGA-Au) --- p.30 / Preparation of the fixative --- p.30 / DNA solution for genotyping of Splotch embryos --- p.31 / Primers used in PCR for genotyping of Splotch embryos --- p.31 / PCR reagent system --- p.32 / 10XTBE --- p.32 / Chapter 2.2 --- Methods --- p.33 / Isolation of embryos from pregnant mice --- p.33 / In situ labelling of exogenous dye --- p.34 / Orthotopical grafting of neural crest fragment --- p.36 / Whole embryo culture --- p.37 / Morphological examination of cultured embryos --- p.38 / Histological examination of cultured embryos --- p.38 / Examination of labelled cells in sectioned embryos --- p.39 / Genotyping of Splotch embryos by PCR --- p.40 / Gel electrophoresis --- p.41 / Chapter CHAPTER THREE --- RESULTS / Chapter 3. 1 --- Initial migration of cardiac neural crest cells in normal ICR mouse embryos --- p.43 / Gross morphological examination of cultured embryos --- p.43 / Distribution of WGA-Au labelled cells in ICR normal mouse embryos --- p.45 / Chapter 3.2 --- Initial migration of cardiac neural crest cells in Splotch embryos --- p.50 / Genotyping --- p.50 / Morphological examination of Splotch mutant embryos --- p.50 / Morphological examination of in vivo Splotch embryos --- p.53 / Distribution of WGA-Au labelled cells in Splotch Embryos --- p.54 / Chapter 3.3 --- Transplantation of neural crest fragments in Splotch embryos --- p.60 / Morphological features of Splotch embryos after orthotopic grafting --- p.60 / Histological examination of Splotch embryos after grafting --- p.61 / Distribution of WGA-Au labelled cells in Splotch embryos after grafting --- p.62 / Chapter CHPATER FOUR --- DISCUSSION / Chapter 4.1 --- Development of embryos in vitro --- p.65 / Chapter 4.2 --- Methodology --- p.70 / In situ labelling of WGA-Au in embryos --- p.70 / Counting of labelled cells in Sploch embryos --- p.72 / Transplantation of neural crest fragments --- p.72 / Chapter 4.3 --- Initial migration of cardiac neural crest cells --- p.74 / Distribution of cardiac neural crest cellsin normal mouse embryos --- p.74 / Differences in the distribution of labelled neural crest cells In different genotypes of Splotch embryos --- p.78 / Distribution of cardiac neural crest cells in Splotch embryos After transplanting of neural crest fragments --- p.83 / Chapter 4.4 --- Factors in extracellular matrix affecting the migration of neural crest cells --- p.88 / Chapter CHAPTER FIVE --- CONCLUSION --- p.91 / REFERENCES --- p.96 / FIGURES AND LEGEND / TABLES / GRAPHS

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323017
Date January 2000
ContributorsCheung, Chui Shan., Chinese University of Hong Kong Graduate School. Division of Anatomy.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 107, [152] leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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