Disease is characterized by signature molecular changes in affected tissues and organs. Diseases cause disruption and dysregulation of a number of biological molecules, including proteins, lipids, metal, and small molecules. Understanding the spatial distribution of biomolecules as it relates to human disease is incredibly important; it represents a way to study disease-associated changes in tissues. This work utilizes Imaging Mass Spectrometry (IMS), a discovery-based analytical approach that enables the detection of biological molecules spatially within diseased tissues, to study infectious diseases. IMS discovered molecular changes specific to areas in the tissue where pathogens interact with their vertebrate hosts. This region, deemed the pathogen-host interface, presents a wealth of information about how vertebrate hosts defend themselves from invading pathogens, including the accumulation and oxidative damage of the metal-chelating host protein calprotectin. In response to metal-starvation, bacteria also exhibit characteristic changes that are observable by mass spectrometry. Further study of these interactions is paramount to the understanding of microbial pathogenesis and to the continued treatment of infectious diseases.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-02282017-115225 |
| Date | 03 March 2017 |
| Creators | Moore, Jessica Lindsey |
| Contributors | Richard Caprioli, Eric Skaar, Walter Chazin, David Cliffel, John McLean |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-02282017-115225/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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