Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005. / Includes bibliographical references. / The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB p50 dimers was assessed. A series of benzophenone-based probes were synthesized and tested for photocrosslinking activity to investigate the efficiency of photocrosslinking in these systems. Three series of small molecule probes were synthesized for the selection of ribozymes from a random sequence pool. Solid-phase immobilized fluorescein and fluorescein phosphates were synthesized for the indirect selection of a fluorescein phosphatase ribozyme. A corresponding thiophosphate analog was created for the in-gel selection of a thiophosphatase ribozyme via APM-PAGE. Finally, a series of fluorescein-nucleoside phosphate conjugates was designed and synthesized for use in the solution phase preparation of a fluorogenic ribozyme substrate, and later immobilization of this substrate on a silyl resin for direct ribozyme selection. / by Jeremy Adam Ryan. / S.M.
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/33657 |
| Date | January 2005 |
| Creators | Ryan, Jeremy Adam |
| Contributors | Alice Y. Ting., Massachusetts Institute of Technology. Dept. of Chemistry., Massachusetts Institute of Technology. Dept. of Chemistry. |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 76 leaves, 4515000 bytes, 4518112 bytes, application/pdf, application/pdf, application/pdf |
| Rights | M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission., http://dspace.mit.edu/handle/1721.1/7582 |
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