Return to search

An investigation of statistical methodologies for evaluating natural herbicides for the control of yellow nutsedge (Cyperus esculentus)

The present study was undertaken with the view to evaluate methodologies based on traditional Scheffé experimental designs that study mixtures as a tool for discovery research particularly when seeking new and or improved uses of existing mixtures. For the purpose of this study, the topic of controlling the problematic weed known as Yellow Nutsedge (Cyperus esculentus L. var. esculentus) or “Geel Uintjie”, was selected on a rather ad hoc basis. Yellow Nutsedge is a troublesome perennial weed found in most agricultural countries in the world. Herbicidal control is often difficult because of the weeds’ ability to propagate via tubers, which can remain dormant for a number of years and are also resistant to most synthetic herbicide controls. As a first step the study involved the selection of a group of chemical compounds that would be used in suppressing the germination of Yellow Nutsedge tubers. Treatment with various combinations of these chemical compounds as determined by statistical experimental designs was carried out. A review of the literature, particularly literature concerned with the study of the phenomenon of allelopathy, suggested that various phenolic-D-glucopyranosides could show promise in the suppressing the germination of Yellow Nutsedge tubers. This led to the selection of this group of compounds as the target group of “active” substances for the study. Since the group of phenolic-D-glucopyranosides is quite large, and in order to keep the study to a reasonable size, only four phenolic-D-glucopyranosides were selected namely: 4-nitrophenyl-D-glucopyranoside, 4-chlorophenyl--Dglucopyranoside, arbutin and salicin. This selection was based firstly based on a particular phenolic-D-glucopyranoside being a suspected allelochemical, and secondly the ease of technical synthesis using a catalytic process. In addition to the four selected phenolic-D-glucopyranosides, their aglycones (4,nitrophenol, 4,chlorophenol, hydroquinone and salicyl alcohol) were also included as potential “active” substances in order to discern any potential activity between the phenolic-D-glucopyranosides and the aglycones. iii The selected “active substances” were combined in various combinations according to various mixture designs in such a manner that the sum of the proportions of the various actives in any one mixture was always equal to 1. The mixtures of actives were then used in various germination experiments and three experimental responses were measured namely the germination, average dry mass and length of longest shoot. From the results of these germination studies the canonical form of the polynomial equation describing the variation in each of the three germination responses was calculated and evaluated statistically. These equations were then used to estimate the presence of, and the magnitude of synergism between the various active substances. The results from these screening experiments and their detailed statistical analysis indicated that the response surface model for the germination response contains three synergistic blends (4-nitrophenyl--D-glucopyranoside + arbutin; 4-nitrophenyl--Dglucopyranoside + hydroquinone; and 4-chlorophenyl--D-glucopyranoside + salicin) and one antagonistic blend (4-nitrophenyl--D-glucopyranoside + 4- chlorophenol--D-glucopyranoside). The response surface model for the average dry mass response contains two synergistic blends (4-nitrophenyl--Dglucopyranoside + hydroquinone; and 4-chlorophenol--D-glucopyranoside + salicin) and the same antagonistic blend as for germination response. For both germination and average dry mass responses, the most synergistic blend was found to be the combination of 4-chlorophenyl--D-glucopyranoside and salicin. Two additional tests were conducted and both confirmed the results obtained from the screening designs. These tests involved the identification of the two components responsible for the synergistic activity that resulted in the suppression of the germination of the tubers and growth of the seedlings. The experimental response measuring the longest shoot proved to be erroneous and was excluded from the statistical analysis. In summary, this study has clearly shown that statistically designed experiments based on mixture designs can be used as a powerful tool in identifying and quantifying synergistic (or antagonistic) effects of chemicals on the germination ability of plant seeds.

  1. vital:10376
Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:nmmu/vital:10376
Date January 2007
CreatorsAsquith, Ilse Bernadette
PublisherNelson Mandela Metropolitan University, Faculty of Science
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeThesis, Doctoral, DTech (Chemistry)
Formatviii, 186 leaves, pdf
RightsNelson Mandela Metropolitan University

Page generated in 0.0018 seconds