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Partial purification and characterization of chitin deacetylase from Mucor rouxii

The optimization of isolation and characterization of chitin deacetylase (CDa) from Mucor rouxii was the focus of this study. The crude extract in the active form was partially purified by ammonium sulfate fractionation, followed by column chromatography by ion exchange in the Fast Protein Liquid Chromatography (FPLC) system. / Using a 10 mM concentration, the order of effectiveness for the inhibitors in achieving 35--40% was CaCl2, CuSO4, MgCl 2 and EDTA. At a 25 mM concentration, propionic acid and sodium acetate inhibited the enzyme up to 40% & 50%, respectively. M. rouxii CDa activity was greater in the mycelial extract, and was capable of efficiently hydrolyzing the substrate glycol chitin. / Two major bands were observed after native PAGE of the partially purified enzyme using Mono Q column (FPLC). The estimated molecular weight of CDa bands by SDS-PAGE containing 0.1% glycol chitin was 21, 23, 26 and 44 kDa when compared to the migration of molecular weight markers. (Abstract shortened by UMI.)

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21548
Date January 1999
CreatorsEltaib, Farag Ibrahim.
ContributorsSimpson, B. K. (advisor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Department of Food Science and Agricultural Chemistry.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001657768, proquestno: MQ50763, Theses scanned by UMI/ProQuest.

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