1 ABSTRACT Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Karolina Palickova Consultant: Ing. Vladimir Kubicek, CSc. Title of Thesis: HPLC Determination of Potencial Drugs The diploma thesis describes selection of the most suitable conditions for HPLC determination of a recently prepared potential bronchodilatans. The aim of the thesis was to find a suitable isocratic method for the HPLC determination of the substance in rat plasma to be used during pharmacokinetic experiments. The chromatographic column Ascentis® Express RP - Amide was utilized. Best results were achieved with mobile phase consisting of acetonitrile and phosphate buffer (pH = 3,0) in volume ratio 12:88. The column was thermostated at 20 řC. Fluorescence detection (λex = 228 nm, λem = 380 nm) was applied to get a sufficient sensitivity. 4-quinazolinole was chosen as an internal standard. Biological samples were processed by LLE before the HPLC analyses. The proposed method was validated successfully and then employed in pharmacokinetic studies.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:368250 |
| Date | January 2017 |
| Creators | Paličková, Karolína |
| Contributors | Kubíček, Vladimír, Drastík, Martin |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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