Return to search

Responses of Cultured Neuronal Networks to the Cannabinoid Mimetic Anandamide

The effects of cannabinoid agonists on spontaneous neuronal network activity were characterized in murine spinal cord and auditory cortical cultures with multichannel extracellular recording using photoetched electrode arrays. Different cultures responded reproducibly with global decreases of spiking and bursting to anandamide and methanandamide, but each agonist showed unique minor effects on network activity. The two tissues responded in a tissue-specific manner. Spontaneous activity in spinal tissue was terminated by 1 μM anandamide and 6.1 μM methanandamide. Cortical activity ceased at 3.5 μM and 2.8 μM respectively. Irreversible cessation of activity was observed beyond 8 μM for both tissues and test substances. Palmitoylethanolamide, demonstrated that CB2 receptors were not present or not responsive. However, the data strongly suggested the presence of CB1 receptors.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc277717
Date05 1900
CreatorsMorefield, Samantha I. (Samantha Irene)
ContributorsGross, Guenter W., Chapman, Kent Dean, Fuchs, Jannon Lou, 1946-
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 73 leaves : ill., Text
RightsPublic, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Morefield, Samantha I. (Samantha Irene)

Page generated in 0.0022 seconds