Effects of carprofen on colon of dog have not been investigated.
Objectives
1) Measure conductance and permeability to mannitol of transverse, proximal descending and distal descending colonic mucosa of dog.
2) Measure conductance and permeability to mannitol of colonic mucosa of dog in presence of carprofen.
Design
In vitro experimental - nested, randomized block design
Animals
Colonic mucosa from 6 (objective 1) and 7 (objective 2) mature mixed-breed dogs.
Methods
Objective 1) Control - Three sections of mucosa from each region of colon were mounted in Ussing chamber units. Conductance was calculated every 15 min for 240 min. Flux of mannitol was calculated for three periods of one hour.
Objective 2) Carprofen - Methods based on results for objective 1. Sections of mucosa were prepared as in objective 1. Carprofen (400μg/ml) was added to bathing solution. Data for conductance and flux of mannitol was obtained as in objective 1.
Histologic examination of all sections was performed after experiment.
For both objectives, conductance was graphed against time for each chamber and area under each curve calculated. Conductance*time, flux of mannitol and frequency distribution of histologic categories were used for analysis.
Results
Objective 1) Mean +/- SEM conductance*time transverse colonic mucosa was higher than proximal and distal descending. Mean +/- SEM flux of mannitol increased from period 1 to period 3 for transverse colonic mucosa. Objective 2) Data from objective 1 was used as control for objective 2. Mean +/- SEM conductance*time carprofen group was higher than control group for all regions of colon. For carprofen group, mean +/- SEM flux of mannitol increased from period 1 to period 2 and from period 2 to period 3 for all regions of colon. There was higher proportion of sections with severe sloughing of cells and erosions involving more than 10% of epithelium in carprofen group compared to control.
Conclusion
Carprofen increases in vitro conductance and permeability to mannitol and causes sloughing of cells and erosions of colonic mucosa of dog which suggests compromise of integrity and loss of barrier function.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-03022007-141419 |
| Date | 04 April 2007 |
| Creators | Briere, Catherine Alix |
| Contributors | Cheryl S. Hedlund, Giselle Hosgood, Rebecca S. McConnico |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-03022007-141419/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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