Adult adipose derived mesenchymal stromal cells (ASCs) have been characterized in various
species. Many factors may affect ASC fate and ASCs from different species may have different
response to these factors. The first study was to identify the differences of the canine ASCs
isolated from subcutaneous and infrapatellar adipose tissues, and evaluate the impact of
cryopreservation on the cells. Based on paired comparisons of fresh and cryopreserved ASCs,
cryopreserved ASCs had lower CD29 and CD44 protein expression and lower proliferation rates.
The cryopreserved ASCs had relative lower mitochondria in the cytoplasm compared to the fresh
ASCs regardless of tissue sources. The second study was to apply human ASCs for bone
regeneration. The spinner flask bioreactor system was employed to load human ASCs onto three
commercial scaffolds and the cell-scaffold constructs were cultured in stromal, osteogenic, or
osteogenic for 48 hours followed by stromal medium for up to 28 days. The distinct scaffold upregulated
different osteogenic signaling pathways, suggesting distinct osteogenic cell signaling
pathways were selectively upregulated by scaffold composition. The third study was designed to
quantify in vivo equine multipotent stromal cell (MSC) osteogenesis on synthetic polymer
scaffolds with distinct mineral combinations 9 weeks after implantation in a murine model.
Addition of mineral to polymer scaffolds enhanced equine MSC osteogenesis over polymer alone,
and contributions by both exo- and endogenous MSCs were confirmed. The fourth study was
designed to evaluate the effects of collagenase digestion and cryopreservation on equine ASCs.
Higher collagenase concentration yielded more nucleated cells, and the percentages of MHCII-,
CD44+, CD105+ cells in freshly isolated and cryopreserved cells were similar. The embryonic
gene expression was enhanced and the essential gene expression decreased after cryopreservation.
The fifth study was to demonstrate the endodermal transdifferentiation capability in feline ASCs.
xvi
Feline-specific pancreatic β cell induction medium was developed in the study, and islet-like cell
clusters that secrete insulin in response to glucose stimulation were created. Overall, the
investigations in this dissertation provide critical information for canine, feline, equine and human
MSC-based tissue engineering therapies and may contribute to better efficiency and efficacy of
cell
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07032017-181133 |
| Date | 10 July 2017 |
| Creators | Duan, Wei |
| Contributors | Lopez, Mandi J., Yao, Shaomian, Andrews, Frank M, Bondioli, Kenneth R |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07032017-181133/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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