Abstract Acetyl-CoA carboxylase (ACC) is a key enzyme of fatty acid metabolism with multiple isozymes often expressed in different eukaryotic cellular compartments. In agriculture, inhibitors of plastid ACC are used as efficient herbicides against grass weed. However, grass weed populations resistant to aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) herbicides represent a major problem for sustainable agriculture. Using PCR and sequencing it was found out that five amino acid substitutions in plastid ACC were correlated with herbicide resistance of Avena sterilis ssp. ludoviciana Durieu populations from the northern grain-growing region of Australia: Trp-1,999-Cys, Trp-2,027- Cys, Ile-2,041-Asn, Asp-2,078-Gly and Gly-2,096-Ala. We showed, using a yeast gene- replacement system, that these single-site mutations also confer herbicide resistance to wheat plastid ACCase: Asp-2,078-Gly confers resistance to APPs and CHDs, Trp-2,027-Cys and Ile-2,041-Asn confer resistance to APPs, and Trp-1,999-Cys confers resistance only to fenoxaprop. These mutations are very likely to confer resistance to any grass weed species under selection imposed by the extensive agricultural use of the herbicides. ...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:330412 |
| Date | January 2012 |
| Creators | Chalupská, Dominika |
| Contributors | Čeřovská, Noemi, Plchová, Helena, Vlček, Čestmír |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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