The herbal preparation coffee charcoal is produced by over-roasting and milling green
dried Coffea arabica L. seeds, and has a long-standing tradition in the treatment of inflammatory and
gastrointestinal disorders. Its therapeutic properties are commonly attributed to adsorptive and
astringent effects. This insufficiently explains its mode of action, especially when used in the treatment
of inflammatory diseases in lower dosages. Our investigations aimed to identify bioactive secondary
plant metabolites affecting cytokine-signaling. Thus, a phytochemical analysis of coffee charcoal
extract was conducted using HPLC and LC/MS. Trigonelline, neochlorogenic acid, chlorogenic
acid, caffeine, cryptochlorogenic acid, feruloylquinic acid isomers, and a caffeoylquinolacton were
identified in the extract. Subsequently, the effects of coffee charcoal extract, chlorogenic acid isomers,
their metabolite caffeic acid, caffeine, and trigonelline on cytokine (TNF, IL-6, MCP-1) release from
LPS-challenged human THP-1 macrophages were examined to evaluate anti-inflammatory activity.
Coffee charcoal showed concentration-dependent mild-to-medium inhibitory effects. The chlorogenic
acid isomers and caffeic acid inhibited the TNF release, with cryptochlorogenic acid exerting the most
distinct effects, as well as decreasing the release of IL-6 and MCP-1. In addition, scanning electron
microscopic images provided an impression of the particle constitution, indicating a larger particle
size and less structured surface of coffee charcoal in comparison to activated charcoal. In conclusion,
our findings underline that beyond adsorptive effects, coffee charcoal exhibits pharmacological
properties, which derive from a spectrum of secondary plant metabolites and support the therapeutic
use in inflammatory diseases. Chlorogenic acids, particularly cryptochlorogenic acid, appear as
pivotal bioactive compounds.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84655 |
| Date | 11 April 2023 |
| Creators | Weber, Laura, Mahdi, Dima Hammoud, Jankuhn, Steffen, Lipowicz, Bartosz, Vissiennon, Cica |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1420-3049, 4263 |
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