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Previous issue date: 2017-03-14 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Fear memories are the main responsible for the defensive behavior expressed in response to threats, however when manifested recurrently and out of context they may develop serious emotional problems, such as post-traumatic stress disorder (PTSD). These memories can be modulated by pharmacological manipulation in the endocannabinoid system and by interaction with other individuals of the same species in addition to methods such as extinction, clinically known as Exposure Therapy, which aims to inhibit the evocation of a previously acquired memory by overlapping a new one. Therefore, the purpose of this study is to demonstrate the effect of social support on the acquisition and extinction of contextual fear conditioning memory (CFC) under the modulation of subtype 1 cannabinoid receptors (CB1). For this, male adult Wistar rats, with bilaterally implanted cannulae in the CA1 region of the dorsal hippocampus by stereotactic surgery were submitted alone or with the presence of a co-specific to 3 minutes of CFC task training, where they received 3 electrical stimuli of 0.5 mA for 2 seconds and 30 seconds interval between each stimulus. At a 24-hour interval, the animals underwent a 3-minute test session to assay memory acquisition, or a 10-minute extinction session and then the test session to assay memory extinction. Furthermore, immediately after the training session, intra-CA1 infusions of saline (1 ?g / ?l), the antagonist (AM251, 100 pmol / ?l) or the agonist (ACEA, 0.01 fmol / ?l) of CB1 receptors were administered. It was found that the presence of a co-specific in the training, extinction or test session induced a significant decrease in the conditioned fear response - measured by the percentage of the animal's immobility time - in all groups, yet even with the expression of memory evocation inhibited by social support, the animals were able to learn the extinction memory. Moreover, a decrease in the conditioned response was observed in the animals receiving both saline and the antagonist infusions, however the greatest effect was observed in the animals receiving agonist infusions, where the inhibition of the response was intensified, presenting the lowest percentages of immobility among all groups. The results obtained in the present study demonstrate the influence of social support on the acquisition and extinction of CFC memory under modulation of CB1 receptors, evidencing its importance on the inhibition of conditioned fear response and facilitation of the memory extinction process, even without the expression of memory evocation along the process. / As mem?rias de medo s?o as principais respons?veis pelo comportamento defensivo expresso em resposta a amea?as, por?m, quando manifestadas de maneira recorrente e fora de contexto, podem desenvolver graves dist?rbios emocionais, como o transtorno do estresse p?s-traum?tico (TEPT). Tais mem?rias podem ser moduladas por manipula??o farmacol?gica no sistema endocanabin?ide e por intera??o com outros indiv?duos da mesma esp?cie, al?m de m?todos como a extin??o, clinicamente conhecida como Terapia de Exposi??o, que visa inibir a evoca??o de uma mem?ria previamente adquirida atrav?s da sobreposi??o de uma nova mem?ria. Com isso, o objetivo deste estudo ? demonstrar o efeito do suporte social sobre a aquisi??o e a extin??o da mem?ria de medo condicionado ao contexto (MCC) sob a modula??o dos receptores canabin?ides do subtipo 1 (CB1). Para isso, ratos Wistar machos adultos, com c?nulas guia implantadas bilateralmente na regi?o CA1 do hipocampo dorsal por meio de cirurgia estereot?xica, foram submetidos sozinhos ou com a presen?a de um co-espec?fico a 3 minutos de treino da tarefa de MCC, onde receberam 3 est?mulos el?tricos de 0,5 mA por 2 segundos e intervalo de 30 segundos entre cada est?mulo. Com um intervalo de 24 horas, os animais foram submetidos a uma sess?o de teste de 3 minutos, para avalia??o da aquisi??o da mem?ria, ou a uma sess?o de extin??o de 10 minutos e ent?o ? sess?o de teste, para avalia??o da extin??o da mem?ria. Al?m disso, imediatamente ap?s a sess?o de treino, foram administradas infus?es intra-CA1 de salina (1 ?g/?l), do antagonista (AM251, 100 pmol/?l) ou do agonista (ACEA, 0.01 fmol/?l) dos receptores CB1. Verificou-se que a presen?a de um co-espec?fico na sess?o de treino, extin??o ou teste induziu uma diminui??o significativa da resposta condicionada de medo ? medida atrav?s da porcentagem do tempo de imobilidade do animal ? em todos os grupos, todavia, mesmo com a express?o da evoca??o da mem?ria inibida pelo suporte social, os animais foram capazes de aprender a mem?ria de extin??o. Ainda, observou-se uma diminui??o da resposta condicionada nos animais que receberam tanto infus?es de salina quanto do antagonista, entretanto, o maior efeito foi observado nos animais que receberam infus?es do agonista, onde a inibi??o da resposta foi intensificada, apresentando as menores porcentagens de imobilidade dentre todos os grupos. Os resultados obtidos no presente trabalho demonstram a influ?ncia do suporte social sobre a aquisi??o e a extin??o da mem?ria de MCC sob modula??o dos receptores CB1, evidenciando sua import?ncia sobre a inibi??o da resposta condicionada de medo e facilita??o do processo de extin??o da mem?ria, mesmo sem a express?o da evoca??o dessa durante o processo.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7399 |
| Date | 14 March 2017 |
| Creators | Ferreira, Fl?via Fagundes |
| Contributors | Izquierdo, Ivan Ant?nio, Myskiw, Jociane de Carvalho |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Gerontologia Biom?dica, PUCRS, Brasil, Instituto de Geriatria e Gerontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 4438661476953179033, 600, 600, 600, 600, 2296420844541114010, -969369452308786627, -2555911436985713659 |
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