Philosophiae Doctor - PhD / Purpose: High risk human papillomavirus (HR-HPV) is well established as a causative agent of squamous
cell carcinoma (SCC) of the orophaynx. HR-HPV has also been reported in periocular cancers and
precancers, but controversy exists about its overall incidence and clinicopathologic profile. The purpose of
this study is to evaluate the role of HR-HPV infection in periocular cancers and precancers, using multiple
methods of detection.
Design: Retrospective observational case series with laboratory investigations.
Methods: Sequential surgical samples of 87 carcinomas (invasive SCC, SCC in situ and sebaceous
carcinoma) from three different periocular sites (conjunctiva, lacrimal sac and the eyelid) diagnosed over a
15-year period (2000-2015) were selected for evaluation. Unstained paraffin sections of 87 cases of
periocular carcinomas were analyzed with immunohistochemistry (IHC) for p16 as a screening test.
p16 positive conjunctival- and lacrimal sac SCC were further evaluated for HR-HPV using DNA in situ
hybridization (DNA ISH), and a subset was also analyzed by DNA Polymerase Chain Reaction (DNA
PCR). p16 positive periocular sebaceous carcinomas (SC) were analyzed with PCR, and a subset of 18cases
was further studied with a novel method of mRNA ISH, an advanced technique with an enhanced sensitivity
and specificity. Relevant patient clinical information was obtained from review of the electronic medical
records.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uwc/oai:etd.uwc.ac.za:11394/6528 |
| Date | January 2018 |
| Creators | Afrogheh, Amir H. |
| Contributors | Hiss, Donavon C. |
| Publisher | University of the Western Cape |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Rights | University of the Western Cape |
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