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Investigation of Copper-Natural Ligand Complexes by RP-HPLC Photodiode Array UV-VIS and Fluorescence Detection

In this study, reversed phase HPLC with dual UV photodiode (PDA) and fluorescence (FL) detection were used to investigate copper complexes with fulvic, caffeic, vanillic, salicylic, and adipic acids. Application of the RE method provided valuable information on the retention behavior and spectral characteristics of FA and model compounds. Even though the method was only applicable to VA, the use of the PDA detector allowed the UV-V is scanning of the separated peaks. This allowed the comparison between the UV-Vis spectra of uncomplexed species. The overall results provide an experimental framework for validation of the proposed Cu-humate interaction models.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc332502
Date08 1900
CreatorsLiao, Jing-Piin
ContributorsWu, Edward Ming-chi, 1938-, Pirtle, Robert M., Masaracchia, Ruthann A., Gracy, Robert W., Jacobson, Myron, Benjamin, Robert C.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatix, 171 leaves : ill., Text
RightsPublic, Liao, Jing-Piin, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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