B cells and in particular IL-10-secreting B cells emerge as important players in immune
balance during pregnancy. We have recently revealed that CD19-deficient (CD19−/−), B cell-specific
IL-10-deficient (BIL-10−/−) and B cell-deficient µMT pregnant mice are highly susceptible to LPSinduced preterm birth (PTB). We aimed to analyze the ability of IL-10-secreting cells to protect from
PTB and the underlying mechanisms. Wild type (WT), CD19−/−, BIL-10−/− and µMT mice were
treated with LPS at gd16 and the cellular immune response was investigated 24 h later. LPS-treated
BIL-10−/− dams showed a more pronounced PTB phenotype compared to WT, CD19−/− and µMT
females, and increased inflammatory and reduced anti-inflammatory mediator concentrations in
the peritoneal cavity and serum. CD19−/−, BIL-10−/− and µMT mice displayed altered immune
cell population frequencies in the blood and uterus with lower numbers of IL-10-secreting B cells
and T cells. BIL-10−/− mothers presented decreased frequencies of uterine CD4+CD25+Foxp3+ Treg
cells. Co-stimulatory molecules are critical for feto-maternal tolerance and IL-10 secretion. We found
dysregulated PD-1 expression in peripheral blood and ICOS expression in the uterus of CD19−/−,
BIL-10−/− and µMT dams. Our data show that B cell-specific IL-10-signaling is essential for a
balanced maternal immune response to an inflammatory stimulant that cannot be hampered without
IL-10-secreting B cells.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88486 |
| Date | 06 December 2023 |
| Creators | Busse, Mandy, Zenclussen, Ana Claudia |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2073-4409, 10.3390/cells11172690 |
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