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Differentiation of Sweet Potato Whitefly Biotypes Using RAPD-PCR

RAPD -PCR was used to detect differences at the DNA level between the A and B forms of B. tabaci. All twenty of the RAPD primers tested distinguished readily between the forms. These primers also distinguished between the forms at the egg and nymph stage. Genetic similarity statistics indicate that these two forms of B. tabaci were no more closely related to each other than to bayberry whitefly (Parabemisia mvricae) or bandedwinged whitefly (Trialeurodes abutilonea). DNA from B. tabaci collected from 11 locations around the world was also analyzed. These insects could be classified into six distinct groups, suggesting there are more than two variant forms of B. tabaci. A comprehensive examination of all variant types of B. tabaci will have to be conducted before a concise definition of the taxonomic relationship between the 'A' and 'B' forms can be determined.

Identiferoai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/209572
Date03 1900
CreatorsGawel, N. J., Bartlett, A. C.
ContributorsSilvertooth, Jeff, Western Cotton Research Laboratory, USDA -ARS, Phoenix, AZ
PublisherCollege of Agriculture, University of Arizona (Tucson, AZ)
Source SetsUniversity of Arizona
Languageen_US
Detected LanguageEnglish
Typetext, Article
Relation370094, Series P-94

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