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pazian_mf_dr_botib.pdf: 2027554 bytes, checksum: 62ff5f16e444d7e659dfe753c88eee67 (MD5) / Foram analisados exemplares de peixes das espécies Characidium cf. fasciatum, C. cf. zebra, C. cf. lagosantense, Characidium sp. e três amostras de C. cf. gomesi, representantes da subfamilia Characidiinae e uma amostra de Crenuchus spilurus, representante de Crenuchiinae, com o uso de técnicas citogenéticas convencionais por coloração com Giemsa, localização das RONs pela impregnação com Nitrato de prata, bandamento C e moleculares, de hibridação in situ fluorescente com sondas de DNAr 18S e 5S, sondas produzidas a partir do cromossomo sexual W e uma sonda produzida a partir do genoma de C. cf. zebra, com ênfase nas sondas produzidas a partir do cromossomo sexual W. Todas as espécies de Characidium apresentaram número diplóide de 50 cromossomos, com predominância de cromossomos dos tipos meta e submetacêntricos, tendo sido observada também a ocorrência de cromossomos supranumerários em uma amostra de C. cf. gomesi e a presença de um sistema de cromossomos sexuais do tipo ZZ-ZW nas espécies Characidium cf. gomesi, Characidium cf. fasciatum, Characidium sp. As espécies Characidium cf. zebra, Characidium cf. lagosantense e Crenuchus spilurus não apresentaram cromossomos sexuais diferenciados. A técnica de pintura cromossômica utilisando sondas produzidas a partir de cromossomos microdissecados e amplificação por DOP-PCR foi realizada para testar a homeologia dos cromossomos sexuais Z e W encontrados em sete amostras de Characidium. Foram produzidas sondas a partir do cromossomo W de Characidium cf. gomesi (DCg) e de Characidium cf. fasciatum (DCf). As sondas mostraram sinais de hibridação nos cromossomos Z e W em Characidium cf. gomesi, Characidium cf. fasciatum, Characidium sp., evidenciando homeologias entre os cromossomos sexuais existentes e uma possível origem comum deste sistema de diferenciação sexual nestas espécies. Por outro lado, em Characidium... / We analyzed samples of fish species Characidium cf. fasciatum, C. cf. zebra, C. cf. lagosantense, Characidium sp. and three strains of C. cf. gomesi, representatives of the subfamily Characidiinae and a sample of Crenuchus spilurus, representative Crenuchiinae, using conventional cytogenetic techniques for staining with Giemsa, location of NORs by impregnation with silver nitrate, C-banding and molecular cytogenetic techniques for fluorescence in situ hybridization with probes of 18S and 5S rDNA, probes produced from the sex chromosome W and a probe generated from the genome of C. cf. zebra, with emphasis on probes produced from the sex chromosome W. All species of Characidium showed a diploid number of 50 chromosomes, with the predominance of chromosomes of metacentric and submetacentric types. The occurrence of supernumerary chromosomes was observed in one sample of C. cf. gomesi and also the presence of a system of sex chromosome ZZ-ZW was observed in Characidium cf. gomesi, Characidium cf. fasciatum, Characidium sp. The species Characidium cf. zebra, Characidium cf. lagosantense and Crenuchus spilurus showed no differentiated sex chromosomes. The technique of chromosome painting using probes produced by microdissected chromosomes amplified by DOP-PCR was performed to test the homeology of Z and W sex chromosomes found in seven species of Characidium. Probes were produced from the W chromosome of Characidium cf. gomesi (DCg) and Characidium cf. fasciatum (DCf). The probes showed hybridization signals on chromosomes Z and W in Characidium cf. gomesi, Characidium cf. fasciatum, Characidium sp., showing homeology between sex chromosomes in the species and a possible common origin of this sexual differentiation system in these species. Moreover, in Characidium cf. zebra, Characidium cf. lagosantense and Crenuchus spilurus the hybridization with ...(Complete abstract click electronic access below)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/106465 |
| Date | 26 March 2013 |
| Creators | Pazian, Marlon Felix [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Foresti, Fausto [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 131 f. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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