This study was undertaken to determine the effect of different concentrations of phosphate, ammonium, nitrate, and sulphate as well as that of shading and moisture stress on oil yield and quality of hydroponically grown rose geranium. Five separate trials were conducted during the 2009 and 2010 growing seasons. Different concentrations of phosphate, ammonium, nitrate and sulphate were used in the first four trials, while the last study focused on the effects of shading and moisture stress on rose geranium.
The phosphate, nitrate, ammonium and sulphate trials were conducted in a greenhouse at the west campus of the University of the Free State in Bloemfontein, South Africa. Plants were grown for four months using a randomized complete block experimental design. The concentrations of phosphorus evaluated were 0.10, 0.80, 1.50 and 2.20 meq L-1. Ammonium concentrations were 0.00, 0.50, 1.00 and 1.50, nitrate concentrations were 8, 10, 12 and 14 and sulphate concentrations were 0.36, 1.90, 3.44 and 4.98 meq L-1.
Foliar drymass and oil yields increased as P concentrations were increased to 2.20 meq L-1. Both, the guaia-6,9-diene content and the citronellol:geraniol (C:G) ratio were better at the high level of phosphate indicating that the best quality oil, as required by the perfume industry is obtained with relatively high phosphate concentrations.
Plant growth as measured by the number of branches and biomass production, peaked at 10 to 12 meq L-1 nitrate concentrations. The highest chlorophyll content in the foliage was found at the nitrate concentrations of 10 and 12 meq L-1, where the best oil yield was also produced. At this nitrate level the citronellol:geraniol (C:G) ratio was slightly higher than the upper limit required for good oil quality but the geraniol and citronellylformate contents were within range for top quality oil. Height, biomass, oil yield and chlorophyll content of the leaves were not affected by ammonium, but the concentrations of plant tissue sulphur and nitrogen increased linearly with increasing concentrations of applied ammonium. Rose geranium needs to be grown at a relatively high nitrate concentration (10 to12 meq L-1) to ensure high oil yield. This application falls within the range that is used for most vegetable and ornamental crops under soilless conditions. Ammonium concentrations of up to 1.00 meq L-1 can be used without affecting yield or oil quality of rose geranium.
A significant effect of sulphate on branches, height and branch:height (B:H) ratio and foliar dry mass (DM) was observed. The four sulphate concentrations showed a statistically non significant trend on yield. Based on the standards used by the perfume industry the oil of rose geranium was not of a good quality in this trial probably due to the autumn planting time.
Shading and moisture stress were used as treatments in a study conducted at the University of the Free State experimental farm during spring and summer. A split plot experimental layout was assigned using 0%, 20%, 40%, 60% and 80% shade treatments allocated to the main plots. The subplots were exposed to moisture stress levels at 0 and -0.15 MPa of osmotic pressure.
Rose geranium grew well under a shading of 40%, where plant growth parameters such as foliar fresh mass (FM), foliar dry mass (DM) and the branch:height ratio were increased. Subsequently the best oil yield was obtained at this level. Proline content was high due to excessive solar radiation at 0% shade as well as where moisture stress was induced, however, oil quality was not affected. The number of oil glands cm-2 of leaf area was not significantly affected by shading, but tended to be lower at shading levels higher than 60%. Fresh mass, DM, the ratio of branches to height and oil yield were affected by shading. Proline content gave a clear indication of stress conditions of plants at full radiation as well as moisture stress. Growers are advised to use 40% shading to grow geraniums in summer at radiation levels similar to those found in this study.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-05172013-123418 |
| Date | 17 May 2013 |
| Creators | Sedibe, Moosa Mahmood |
| Contributors | Dr GM Engelbrecht, Dr J Allemann |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-05172013-123418/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
Page generated in 0.0198 seconds