Plant viruses have shown to be the cause of the yellow vein symptoms on the foliage of several ornamental plants. In this study five ornamental plants: oxalis (Oxalis debilis var. corymbosa) cv. Golden Veined Oxalis, geranium (Pelargonium peltatum) cv. Crocodile, pseuderanthemum (Pseuderanthemum carruthersii) cv. Golden Net Bush (narrow leaf and broad leaf variants), honeysuckle (Lonicera japonica) cv. Yellow Net Honeysuckle and coleus (Solenostemon scutellarioides) cv. Electric Lime, showing foliar yellow vein were used. Graft transmissions indicated that, except for coleus, the foliar yellow vein of all other plants was caused by infectious agents, likely plant viruses. With the exception of Golden Veined Oxalis, attempts to identify a virus as the causal agent of the foliar yellow vein in geranium, pseuderanthemum, and honeysuckle failed.
Successful whitefly (Bemisia tabaci biotype B) transmission of the foliar yellow vein of Golden Veined Oxalis suggested that a begomovirus was the causal agent. Therefore, PCR amplifications were conducted using degenerate primers for the genus Begomovirus. A 1.2 kb PCR product was obtained using the primer set PAL1v1978/ PAR1c496 and a 0.7 kb product with the primer set AV494/AC1048. These sets of primers have been used widely to detect and identify begomoviruses. Sequencing of the fragments confirmed the begomovirus nature of the amplified DNA from Golden Veined Oxalis. Sequence information and phylogenetic analyses revealed that the Golden Veined Oxalis begomovirus was closely related to Tomato yellow spot virus and Sida mottle virus, two begomoviruses reported to infect solanaceous and legume crops. The host range of the virus isolated from Golden Veined Oxalis begomovirus, designated Oxalis yellow vein virus, is not known. This finding illustrates the potential spread of plant viruses to different geographical areas through the commercialization of virus- infected ornamental plants.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-04112014-183528 |
| Date | 01 May 2014 |
| Creators | Herrera, Favio Eduardo |
| Contributors | Bush, Edward, Singh, Raghuwinder, Clark, Christopher, Valverde, Rodrigo |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-04112014-183528/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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