Bacterial panicle blight is among the three most limiting diseases of rice production in the United States. Yield loss up to 40% has been reported from rice fields severely infested with bacterial panicle blight. The disease is caused by Burkholderia glumae and B. gladioli. Although, both species produce similar symptoms and are closely related, but B. glumae strains are more aggressive and cause more severe symptoms on rice. Bacterial panicle bight is difficult to manage in the absence of effective chemical control measures. Rapid and early disease detection is needed to avoid severe yield losses. Several techniques have been developed for bacterial identification, but these methods are time consuming and some require high-precision instruments for amplification and analysis of target DNA. We developed a Loop-Mediated Isothermal Amplification (LAMP) protocol for rapid detection of B. glumae using a set of six primers from the gyrB housekeeping gene. Several commercially available dyes including, PicoGreen, Hydroxynaphthol Blue (HNB) and Calcein were compared to analyze the LAMP product. The LAMP detection method resulted in rapid and accurate detection of B. glumae. Among the different detection dyes, PicoGreen and HNB produced reliable results in the detection of B. glumae. Although, both produced accurate results; however, HNB is more cost-effective.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-06092014-145316 |
| Date | 21 July 2014 |
| Creators | Caldera, Maria Alejandra |
| Contributors | Singh, Raghuwinder, Ham, Jong Hyun, Groth, Donald |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-06092014-145316/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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