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Effects of Escapin Intermediate Products (EIP-K) on Biofilms of Pseudomonas aeruginosa

Escapin is an L-amino acid oxidase that produces antimicrobial metabolites collectively called “Escapin Intermediate Products” (EIP-K). EIP-K and H2O2 together were previously shown to be bactericidal towards diverse planktonic bacteria. The present work investigates the ability of EIP-K and H2O2 to antagonize bacterial biofilms, using Pseudomonas aeruginosa as a model. The project had three aims: 1) determine the most effective concentrations of EIP-K and H2O2 necessary to break down existing P. aeruginosa biofilms, using a crystal violet assay; 2) examine the ability of EIP-K + H2O2 to inhibit biofilm formation, using triphenyl tetrazolium chloride dye; and 3) determine the effect of EIP-K + H2O2 on the viability, biomass and structure of biofilms cultivated in flow cells using confocal laser scanning microscopy (CLSM). Results showed that EIP-K + H2O2 significantly reduced biofilm biomass relative to controls and that the compounds are effective at nanomolar concentrations.

Identiferoai:union.ndltd.org:GEORGIA/oai:digitalarchive.gsu.edu:biology_theses-1050
Date03 August 2013
CreatorsAbdelaziz Ahmed, Marwa Nabil
PublisherDigital Archive @ GSU
Source SetsGeorgia State University
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceBiology Theses

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