Sweet potato SPCP2 is a full-length cDNA isolated from senescent leaves and encodes a putative papain-like cysteine protease. The SPCP2 contained 1101 nucleotides (366 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 68% to 83%) with plant cysteine proteases, including Actinidia deliciosa, Arabidopsis thaliana, Brassica oleracea, Phaseolus vulgaris, Pisum sativa, Vicia faba, Vicia sativa and Vigna mungo. SPCP2 gene expression was enhanced significantly in natural senescent leaves and in sprouting storage root. Transgenic Arabidopsis plants with ectopic constitutive SPCP2 expression showed earlier floral transition from vegetative to reproductive growth, reduced rosette leaves when flowering, enhanced germination percentage of transgenic progeny seeds in salt-containing MS medium, higher Fv/Fm value, higher relative water content and enhanced tolerance during drought treatment. Based on these results, we conclude that sweet potato papain-like cysteine protease, SPCP2, is a functional gene, and its expression causes altered developmental characteristics and enhances drought and salt stress responses in transgenic Arabidopsis plants.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0617110-084745 |
| Date | 17 June 2010 |
| Creators | Lin, Chia-hung |
| Contributors | Chung-Lung Cho, Hsien-Jung Chen, Guan-jhong Huang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745 |
| Rights | campus_withheld, Copyright information available at source archive |
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