Accurate chromosome segregation during meiosis is critical for generating genetic diversity and for producing gametes with the correct number of chromosomes. After meiotic S-phase, homologs pair, recombine, and then separate in meiosis I. Meanwhile sister chromatids remain cohesive until meiosis II. A tripartite cohesin ring made of Smc1, Smc3, and the klesin subunit (Rec8 in meiosis and Mcd1/Scc1 in mitosis) is thought to topologically entrap a pair of sister chromatids generating cohesion. The role of the fourth cohesin subunit, Scc3, remains elusive. To study the function of Scc3 in yeast meiosis, we constructed PCLB2SCC3, a meiosis-specific mutant in which the expression of Scc3 is activated in mitosis but is suppressed in meiosis. With the mutant, we first found that Scc3 is required for sister chromatid cohesion and nuclear division during meiosis in the budding yeast, Sacchromyces cerevisiae. Second, we found that Scc3 is also required for maintaining Rec8 protein level during meiosis, which is independent of the Scc3's function in sister chromatid cohesion and nuclear division. Therefore, we asked how Scc3 regulates Rec8 protein levels. Our results demonstrate that Scc3 regulates neither REC8 mRNA production nor Rec8 protein stability, which suggestes that Scc3 have a role in posttranscriptional regulation in REC8 expression. At last, Scc3 is essential for Rec8−chromosome binding, whereas Rec8 only partly regulates Scc3−chromosome binding. Our data provide molecular insights into the role Scc3 in accurate chromosome segregation during meiosis. / A Thesis submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Master of Science. / Summer Semester, 2010. / May 3, 2010. / cohesion, meiosis, yeast / Includes bibliographical references. / Hongguo Yu, Professor Directing Thesis; Yanchang Wang, Committee Member; George W. Bates, Committee Member; Hank W. Bass, Committee Member.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_182711 |
| Contributors | Wang, Mian (authoraut), Yu, Hongguo (professor directing thesis), Wang, Yanchang (committee member), Bates, George W. (committee member), Bass, Hank W. (committee member), Department of Biological Science (degree granting department), Florida State University (degree granting institution) |
| Publisher | Florida State University, Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text |
| Format | 1 online resource, computer, application/pdf |
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