This thesis will discuss the 'Polima' cytoplasmic male sterility (CMS) system (pol) in Brassica napus (Canola) and detailed genetic mapping of the region surrounding restorer gene ( Rfp) for that system This fine mapping of the Rfp region will facilitate efforts to clone the gene that will eventually lead to its characterization. Knowledge of the structure of Rfp will provide insight in the molecular mechanisms governing mitochondrial gene statement as well as pollen production and may lead to the development of alternative methods of pollination control. In addition, it is possible that nuclear restorer genes for other CMS systems in other crops may be similar to that of the 'Polima' system. / Map based cloning requires the identification of DNA markers tightly linked to Rfp. Two PCR based markers which are located on either side of Rfp were developed. These markers allowed facile screening of a large population. / RFLP markers used in this study are based on the synteny between B. napus and the well known crucifer A. thaliana. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.34014 |
| Date | January 2001 |
| Creators | Stollar, Rachel. |
| Contributors | Brown, Gereg (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001872991, proquestno: MQ79116, Theses scanned by UMI/ProQuest. |
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