Rye grass are common considered optimum grass species for intensive agriculture in area with sufficient rainfall, because they provide a high production of quality forage. Unfortunately, they have lack of resistance to abiotic stresses. With heed to the progress climate change, when these stresses will increase, we are looking for genes from better-adapted species of Festuca. Target features are better use of nitrogen and water to attainment high production of forage, increased tolerance to drought and ability rooting compacted layers of soil. These targets lead to research on growth of roots, which are to fulfil the above written targets in award. Following the research of M. Humphreys we are focused on detailed monitoring root growth of three species xFestulolium and their parent species in the early stages of development in our research. They were tested following grasses: Festuca arundinacea (cv. KORA), Festuca pratensis (cv. PRONELA), Lolium multiflorum (cv. MORAVICE) and xFestulolium (cv. HYKOR, HOSTYN and PRIOR). The chosen the method of cultivation in cylinder with sand in outdoor conditions with fertilization in two doses, corresponding to a total 80 kg N/ha. The experiment was performed in two terms: 21. 5. when 23. 7. 2015 and 21. 9. when 11. 12. 2015. In the first term, growth was limited by high temperatures. In the second term were limiting the contrary low temperatures, especially at the end of vegetation. The evaluated parameters were: maximum depth of rooting, total dry root biomass, total root length, root surface, root volume, the ratio R/S, the dry weight of aboveground biomass, maximum height aboveground biomass, number of tillers, number developed leaves, surfaces of the leaves and root length in individual thickness categories. The length, surface and volume of roots with flat leaves, were evaluated through image analysis scans in program WinRHIZO (Regent Instruments, Canada). In most parameters it was the best Lolium multiflorum MORAVICE with xFestulolium HOSTYN. The differences between them are in most of parameters statistically insignificant (outside the maximum depth of root and flat of leaves, where MORAVICE achieved significantly higher values). Anticipated advantages relating to the growth of strong and deep roots in this experiment did not show. The supposed main reason for the termination of the experiment in the early stage of development.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:362634 |
| Date | January 2016 |
| Creators | Lukášek, Dominik |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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