Chu Wai-ling. / Thesis submitted in: December 2003. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 106-114). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract (Chinese) --- p.iii / Acknowledgments --- p.v / Contents --- p.vi / List of Tables --- p.ix / List of Figures --- p.x / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter Chapter 2 --- Literature Review --- p.8 / Chapter 2.1 --- Introduction to phylogenetic biology --- p.8 / Chapter 2.1.1 --- Definition of phylogenetics --- p.8 / Chapter 2.1.2 --- Why employ molecular genetic markers in phylogenetics? --- p.8 / Chapter 2.2 --- DNA analysis and the contributions to phylogenetics --- p.10 / Chapter 2.2.1 --- Historical development of DNA analysis in phylogenetics --- p.10 / Chapter 2.2.2 --- Nuclear ribosomal DNA (rDNA) --- p.12 / Chapter 2.2.3 --- Animal mitochondrial DNA (mt DNA) --- p.14 / Chapter 2.3 --- Molecular phylogeny of crustaceans --- p.16 / Chapter 2.3.1 --- Phylogenetic studies of crustaceans using nuclear ribosomal DNA --- p.16 / Chapter 2.3.2 --- Phylogenetic studies of crustaceans using mitochondrial DNA --- p.17 / Chapter 2.4 --- Taxonomy of the genus Heterocarpus --- p.22 / Chapter Chapter 3 --- Materials and Methods --- p.36 / Chapter 3.1 --- Collection and storage of specimens --- p.36 / Chapter 3.2 --- DNA extraction --- p.36 / Chapter 3.3 --- Amplification of mitochondrial genes --- p.38 / Chapter 3.3.1 --- PCR profile --- p.39 / Chapter 3.3.1.1 --- 16SrRNA gene --- p.39 / Chapter 3.3.1.2 --- COI gene --- p.42 / Chapter 3.3.1.2.1 --- Amplification of COI gene segments using primers LCD1490/HCO2198 --- p.42 / Chapter 3.3.1.2.2 --- Amplification of COI gene segments using primers COIf/COIa and COIp3/COIa --- p.43 / Chapter 3.4 --- DNA sequencing --- p.44 / Chapter 3.4.1 --- Purification of extension products --- p.45 / Chapter 3.4.2 --- Electrophoresis and data collection --- p.46 / Chapter 3.5 --- Data analysis --- p.47 / Chapter Chapter 4 --- Results --- p.50 / Chapter 4.1 --- PCR products of 16S rRNA and COI genes --- p.50 / Chapter 4.2 --- Genetic variability in Heterocarpus based on partial DNA sequence of 16S rRNA gene --- p.52 / Chapter 4.3 --- Genetic variability in Heterocarpus based on COI gene --- p.61 / Chapter 4.3.1 --- Genetic variability in Heterocarpus based on partial DNA sequence of COI gene --- p.61 / Chapter 4.3.2 --- Genetic variability in Heterocarpus based on amino acid sequence of COI --- p.69 / Chapter 4.4 --- Phylogenetic analysis --- p.75 / Chapter 4.4.1 --- Phylogenetic analysis based on 16S rDNA sequence --- p.75 / Chapter 4.4.2 --- Phylogenetic analysis based on DNA sequence of COI gene --- p.80 / Chapter 4.4.3 --- Phylogenetic analysis based on amino acid sequence of COI --- p.84 / Chapter 4.5 --- Kishino-Hasegawa and Shimodaira-Hasegawa tests --- p.86 / Chapter Chapter 5 --- Discussion --- p.90 / Chapter 5.1 --- Examination on the validity of the four Heterocarpus complexes --- p.90 / Chapter 5.2 --- Phylogenetic relationship of Heterocarpus species within each complex --- p.91 / Chapter 5.2.1 --- Phylogenetic relationship of Heterocarpus species within H.gibbosus complex --- p.92 / Chapter 5.2.2 --- Phylogenetic relationship of Heterocarpus species within H.woodmasoni complex --- p.94 / Chapter 5.2.3 --- Phylogenetic relationship of Heterocarpus species within H. ensifer and H. sibogae complexes --- p.96 / Chapter 5.3 --- Phylogenetic relationship among Heterocarpus complexes --- p.98 / Chapter 5.4 --- "Comparisons of phylogenetic resolving power of 16S rRNA, COI and 28S rRNA genes" --- p.100 / Chapter Chapter 6 --- Conclusions --- p.104 / Literature Cited --- p.106
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324955 |
| Date | January 2004 |
| Contributors | Chu, Wai-ling., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xi, 114 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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