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Previous issue date: 2012 / O papilomavírus humano (HPV) é o principal agente etiológico de lesões
malignas, a exemplo do câncer cervical e de suas lesões precursoras, as Neoplasias
Intraepiteliais Cervicais (NICs). Investigamos se os polimorfismos de base única (SNPs)
no gene da Beta-Defensina Humana 1 (DEFB1) poderiam estar associados à
susceptibilidade à infecção e à progressão da lesão cervical provocadas por HPV de alto
risco. Polimorfismos neste gene têm sido alvo de diversos estudos e revelaram ser de
suma importância na susceptibilidade a diversas infecções. Neste estudo, os SNPs
-52G/A, -44C/G e -20 G/A na região 5' UTR do gene DEFB1 foram analisados através de
sequenciamento, em um grupo de 160 pacientes do sexo feminino HPV positivas e 71
indivíduos sadios. As análises estatísticas foram feitas utilizando o Teste exato de Fisher e
o Teste do Qui-quadrado. Os resultados indicam que o alelo -44G e o haplótipo ACA
podem estar relacionados com a susceptibilidade à infecção pelo HPV de alto risco. Os
resultados não apontam diferenças significativas nas frequências alélicas e genotípicas
dos outros polimorfismos entre pacientes infectados com HPV de alto risco e indivíduos
sadios. Também não foram encontradas diferenças estatisticamente significativas nas
frequências alélicas e genotípicas nos pacientes HPV positivos quanto ao grau de lesão
cervical. / Human papillomavirus (HPV) is the main etiological agent for development of
cervical cancer and its precursor lesions, the cervical intraepithelial neoplasia (CINs). We
investigated whether single-nucleotide polymorphisms (SNPs) in the human Beta-Defensin
1 gene (DEFB1) could be associated with susceptibility to infection and progression of
cervical lesions caused by high-risk HPV. Polymorphisms in this gene have been the
subject of several studies and proved to be extremely important in susceptibility to various
infections. In this study, SNPs -52 G/A, -44 C/G and -20 G/A in the 5 'UTR of DEFB1 were
analyzed by direct sequencing in a group of 160 HPV-positive female patients and 71
healthy individuals. Statistical analysis was performed using Fisher's exact Test and Chisquare.
The results indicate that the -44G allele and haplotype ACA may be associated
with susceptibility to infection caused by high-risk HPV. The results show no significant
differences in allelic and genotypic frequencies of the other polymorphisms in patients
infected with high-risk HPV and healthy individuals. We also found no statistically
significant differences in allelic and genotypic frequencies in HPV-positive patients related
to the progression of cervical lesion.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpe.br:123456789/18513 |
| Date | January 2012 |
| Creators | SAUVÉ, Jean Phillippe Guimarães |
| Contributors | CROVELLA, Sergio, SOUZA, Paulo Roberto Eleutério de |
| Publisher | Universidade Federal de Pernambuco, Programa de Pos Graduacao em Genetica, UFPE, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Breton |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFPE, instname:Universidade Federal de Pernambuco, instacron:UFPE |
| Rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil, http://creativecommons.org/licenses/by-nc-nd/3.0/br/, info:eu-repo/semantics/openAccess |
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