A series of four experiments was undertaken to determine both the quantitative and qualitative effects of magnesium deprivation on the urinary glycoproteins of adult and grow.ing rats. The glycoproteins were to be isolated in 0.58 M Na.Cl, the isolation technique for Tamm-Horsfall glycoprotein. Quantitative excretion was measured as ɣ hexose/rat/day. Qualitative analyses were reported as percent of dry weight of material isolated.
A glycoprotein-containing material precipitated spontaneously from the urine before addition of NaCl. The dry weight ratio of spontaneously precipitating material (Fraction I) to salt-precipitable material (Fraction II) was 16 to one in normal animals, 3.4 to one in depleted animals. The hexose to amino acid to uronic acids ratio was the same for both fractions in both groups. No hexosamines were found in either fraction. The ash content was lower in Fraction I for deficient animals, but higher in Fraction II for deficient animals, when compared to control animals. Increased phosphate binding by Fraction II from deficient animals was indicated.
A slight rise in total glycoprotein excreted daily was shown in animals fed a magnesium deficient diet, but not until after the first week, when irreversible kidney damage is initiated. Control animals whose weight ga:m was restricted to 32% 0£ normal excreted less of Fraction I per day. Amino acid patterns of both fractions from all groups were similar, but differed from those reported for human and sheep Tamm-Horsfall glycoprotein. All depleted animals showed a significant (p> 0.01} increase in kidney calcium content, and one group showed a significant (p> 0.01) decrease in kidney magnesium content at five weeks. / M.S.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/90976 |
| Date | January 1968 |
| Creators | Poe, Clyde Douglas |
| Contributors | Biochemistry |
| Publisher | Virginia Polytechnic Institute |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | en_US |
| Detected Language | English |
| Type | Thesis, Text |
| Format | iv, 52, [2] leaves, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 2284670 |
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