ABSTRACT
Deinococcus radiodurans is extremely resistant to ionizing and UV irradiation. The addition of manganese(¢º) into stationary-phase culture can trigger a new cycle of cell division ( Mn-CD effect ). Although D.radiodurans can hardly utilize sugar , but it does can incorporate glucose and fructose into the cell mass. We found that D. radiodurans can metabolize sugar by glycolysis ( EMP ) and pantose phosphate pathway ( PPP ). The activity of both pathways would be altered by the addition of manganese(¢º). The marker enzymes of the PPP ( transketolase ) and EMP pathway ( aldolase ) were also analyzed. Results show that manganese(¢º) can induce the hydrolysis of glucose by activating the EMP pathway. However , the induction effect various with different cultural conditions. In analyzing the PPP pathway , the activities of transketolase were also various with different cultural conditions.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0219101-094458 |
| Date | 19 February 2001 |
| Creators | Hsueh, Ya-chao |
| Contributors | Chi-Hsin Hsu, Chan-Shing Lin, Jong-Kang Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219101-094458 |
| Rights | unrestricted, Copyright information available at source archive |
Page generated in 0.0015 seconds