Abstract
The highly radioresistant Deinococcus radiodurans is a heterotrophic Gram positive aerobic tetrad. The addition of 2.5 uM Mn(II) into the early stationary-phase culture would trigger a new round of cell division (Mn-CD effect) which is specific to this unique bacterium. In order to understand the reactions of this bacterium to different carbohydrates, we culture the bacterium by using complex media with various monosaccharides and disaccharides.
No matter in the present of Mn(II) or not, the specific growth rate of fructose¡Vgrown cells was always higher than those of glucose-grown cells. Cell growth would be inhabited by excess Mn(II). Along with the increasing of Mn(II), the culture showed a prolonged lag phase. In chemically defined media, this bacterium grew well by using glucose, fructose, mannose, maltose or sucrose as it¡¦s sole carbon source and performed the Mn-CD growth by adding Mn(II).
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Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0302101-162841 |
Date | 02 March 2001 |
Creators | Chen, Jean-Lin |
Contributors | Chang-Sing Lin, Jong-Ken Liu, J-Jing Sue |
Publisher | NSYSU |
Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
Language | Cholon |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0302101-162841 |
Rights | unrestricted, Copyright information available at source archive |
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